Discovery of surface target proteins linking drugs, molecular markers, gene regulation, protein networks, and disease by using a web-based platform targets-search / Bin Yan, Panwen Wang, Junwen Wang, and Kenneth R. Boheler. "Shaving" live bacterial cells with proteases for proteomic analysis of surface proteins / Manuel J. Rodrıguez-Ortega -- Methods for mapping the extracellular and membrane proteome in the avian embryo, and identification of putative vascular targets or endothelial genes / Witold W. Kilarski, John Herbert, and Andreas Bikfalvi -- Mass spectrometry-based identification of extracellular domains of cell surface N-glycoproteins : defining the accessible surfaceome for immunophenotyping stem cells and their derivatives / Chelsea M. Fujinaka, Matthew Waas, and Rebekah L. Gundry -- Application of higher density iron oxide nanoparticle pellicles to enrich the plasma membrane and its proteome from cells in suspension / Rebecca L. Rose, Waeowalee Choksawangkarn, and Catherine Fenselau -- Proteomic profiling of secreted proteins, exosomes, and microvesicles in cell culture conditioned media / Ankit Sinha, Simona Principe, Javier Alfaro, Alex Ignatchenko, Vladimir Ignatchenko, and Thomas Kislinger -- Cloning, expression, and purification of the glycosylated transmembrane protein, cation-dependent mannose 6-phosphate receptor, from Sf9 cells using the baculovirus system / Linda J. Olson and Nancy M. Dahms -- Bispecific antibody armed T cells to target cancer cells / Archana Thakur, Lawrence G. Lum, and Sandeep Mittal -- Immunophenotyping of live human pluripotent stem cells by flow cytometry / Daniel R. Riordon and Kenneth R. Boheler -- Detecting cell surface expression of the G protein-coupled receptor CXCR4 / Amanda M. Nevins and Adriano Marchese -- NaV channels : assaying biosynthesis, trafficking, function / Gordon F. Tomaselli and Federica Farinelli -- High-content electrophysiological analysis of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) / Chi-Wing Kong, Lin Geng, and Ronald A. Li -- Methods for evaluation of vascular endothelial cell function with transient receptor potential (TRP) channel drugs / Yung Wui Tjong and Xiaoqiang Yao -- Methods to study the signal transduction of the surface receptor tyrosine kinase TrkB in neurons / Kwok-On Lai and Nancy Y. Ip -- Polarized human retinal pigment epithelium exhibits distinct surface proteome on apical and basal plasma membranes / Vladimir Khristov, Qin Wan, Ruchi Sharma, Mostafa Lotfi, Arvydas Maminishkis, and Kapil Bharti -- Extracellular matrix molecule-based capture of mesenchymal stromal cells under flow / Teresa Massam-Wu, Stuart A. Cain, and Cay M. Kielty -- Generation of induced pluripotent stem cells from patients with COL3A1 mutations and differentiation to smooth muscle cells for ECM-surfaceome analyses / Jiaozi He, Zhihui Weng, Stanley Chun Ming Wu, and Kenneth R. Boheler -- Fabrication and mechanical properties measurements of 3D microtissues for the study of cell-matrix interactions / Prasenjit Bose, Chen Yu Huang, Jeroen Eyckmans, Christopher S. Chen, and Daniel H. Reich -- Discovery of surface target proteins linking drugs, molecular markers, gene regulation, protein networks, and disease by using a web-based platform targets-search / Bin Yan, Panwen Wang, Junwen Wang, and Kenneth R. Boheler.
Summary:
This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches for surfaceome content; cell-based function analyses related to surfaceome content; and computational approaches in surfaceome studies. Section 1 focuses on discovery-based approaches for cataloging surfaceome content that analyses the surfaceome of bacteria, avian embryos, and mammalian systems. Section 2 discusses methods that over-express specific targets in Sf9 cells and generate bi-specific antibodies for targeting cancer and somatic cells. Section 3 explores voltage dependent sodium channels and high-content electrophysiological analyses. The final section looks at the new web-based platform known as targets-search. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Surfaceome: Methods and Protocols assists in the study of cell surface protein biology and function. It is a valuable resource for all researchers interested in this field.
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.