505-00 Manipulation and measurement of AMPK activity in pancreatic islets / Aida Martinez-Sanchez, Marie-Sophie Nguyen-Tu, Isabelle Leclerc, and Guy A. Rutter. Visualizing AMPK drug binding sites through crystallization of full-length phosphorylated α2β1γ1 heterotrimer / Christopher G. Langendorf, Jonathan S. Oakhill, and Bruce E. Kemp -- Biophysical interactions of direct AMPK activators / Ravi G. Kurumbail, Graham M. West, Venkatasubramanian Dharmarajan, Kris A. Borzilleri, Jane M. Withka, Jessica Ward, Allan R. Reyes, Francis Rajamohan, Patrick R. Griffin, and Matthew F. Calabrese -- Biochemical measurement of glycogen : method to investigate the AMPK-glycogen relationship / Elite Possik and Arnim Pause -- Cell-free assays to measure effects of regulatory ligands on AMPK / Fiona A. Fyffe, Simon A. Hawley, Alexander Gray, and D. Grahame Hardie -- Applications of NMR and ITC for the study of the kinetics of carbohydrate binding by AMPK β-subunit carbohydrate-binding modules / Paul R. Gooley, Ann Koay, and Jesse I. Mobbs -- Bioinformatics approach to identify novel AMPK targets / Brendan Gongol, Traci Marin, David A. Johnson, and John Y. -J. Shyy -- Studying AMPK in an evolutionary context / Arpit Jain, Valentin Roustan, Wolfram Weckwerth, and Ingo Ebersberger -- AMPK protein interaction analyses by yeast two-hybrid / Pascual Sanz, Rosa Viana, and Maria Adelaida Garcia-Gimeno -- Transient expression of AMPK heterotrimer complexes in mammalian cells / Jonathan S. Oakhill, John W. Scott, and Toby A. Dite -- Knockdown of human AMPK using the CRISPR/Cas9 genome-editing system / Adrien Grenier, Pierre Sujobert, Severine Olivier, Helene Guermouche, Johanna Mondesir, Olivier Kosmider, Benoit Viollet, and Jerome Tamburini -- Compound C/Dorsomorphin : its use and misuse as an AMPK inhibitor / Biplab Dasgupta and William Seibel -- Identifying the heterotrimeric complex stoichiometry of AMPK in skeletal muscle by immunoprecipitation / Jesper B. Birk and Jørgen F. P. Wojtaszewski -- Kinase activity determination of specific AMPK complexes/heterotrimers in the skeletal muscle / Jesper B. Birk and Jørgen F. P. Wojtaszewski -- Determination of adenine nucleotide concentrations in cells and tissues by high-performance liquid chromatography / Noemi Garcia-Tard on and Bruno Guigas -- Intact cell assays to monitor AMPK and determine the contribution of the AMP-binding or ADaM sites to activation / Simon A. Hawley, Fiona A. Fyffe, Fiona M. Russell, Graeme J. Gowans, and D. Grahame Hardie -- Cellular application of genetically encoded sensors and impeders of AMPK / Takafumi Miyamoto, Elmer Rho, Allen Kim, and Takanari Inoue -- Assessing mitochondrial bioenergetics by respirometry in cells or isolated organelles / Guillaume Vial and Bruno Guigas -- Study of AMPK-regulated metabolic fluxes in neurons using the seahorse XFe analyzer / Claudia Marinangeli, Jerome Kluza, Philippe Marchetti, Luc Buee, and Valerie Vingtdeux -- Investigating the role of AMPK in inflammation / Sarah J. Mancini and Ian P. Salt -- Studying the role of AMPK in cardiac hypertrophy and protein synthesis / Florence Mailleux, Christophe Beauloye, Jean-Luc Balligand, Sandrine Horman, and Luc Bertrand -- Assessment of AMPK-stimulated cellular long-chain fatty acid and glucose uptake / Joost J. F. P. Luiken, Dietbert Neumann, Jan F. C. Glatz, Will A. Coumans, Dipanjan Chanda, and Miranda Nabben -- Measurement of AMPK-induced inhibition of lipid synthesis flux in cultured cells / Marc Foretz and Benoit Viollet -- Studying the role of AMPK in autophagy / Sarah Krieg, Bernhard Luscher, Jorg Vervoorts, and Marc Dohmen -- Determining AMPK activation via thelysosomal v-ATPase-ragulator-AXIN/LKB1 axis / Chen-Song Zhang, Mengqi Li, Yue Zong, and Sheng-Cai Lin -- Manipulation and measurement of AMPK activity in pancreatic islets / Aida Martinez-Sanchez, Marie-Sophie Nguyen-Tu, Isabelle Leclerc, and Guy A. Rutter.
Summary:
520-00 This volume explores the latest methods used to study AMPK by computational, biochemical, biophysical, cellular, and ex vivo and in vivo approaches. The chapters in this book cover specific topics, such as methods to measure change in cellular energy metabolism and analyze metabolic pathways regulated by AMPK; bioinformatics tools to identify AMPK targets; knockdown of AMPK by CRISPR-Cas9; production and crystallization of full-length human AMP-activated protein kinase; cell-free assays to measure the effects of regulatory ligands on AMPK; use of sensors of AMPK activity; AMPK protein interaction by yeast two-hybrid; the role of AMPK in inflammation and autophagy; analyzing the AMPK function in C. elegans and mammals (with special focus on skeletal muscle, blood vessels, kidneys, pancreatic islets and hypothalamus); and human γ2 AMPK mutations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, AMPK: Methods and Protocols is a valuable resource for graduate students, postdoctoral researchers, and established investigators who are interested in the richly diverse AMPK field.
Series:
Methods in molecular biology, 1064-3745 ; 1732 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.