Looking for broken TAD boundaries and changes on DNA interactions : clinical guide to 3D chromatin change analysis in complex chromosomal rearrangements and chromothripsis / Kevin Yauy, Vincent Gatinois, Thomas Guignard, Satish Sati, Jacques Puechberty, Jean Baptiste Gaillard, Anouck Schneider, and Franck Pellestor. Clinical consequences of chromothripsis and other catastrophic cellular events / Maki Fukami and Hiroki Kurahashi -- Potential role of chromothripsis in the genesis of complex chromosomal rearrangements in human gametes and preimplantation embryo / Franck Pellestor and Vincent Gatinois -- Chromothripsis and the macroevolution theory / Franck Pellestor -- Analysis of chromothripsis by combined FISH and microarray analysis / Ruth N. MacKinnon -- Chromothripsis detectable in small supernumerary marker chromosomes (sSMC) using fluorescence in situ hybridization (FISH) / Thomas Liehr -- Identification of chromothripsis in biopsy using SNP-based microarray / Veronica Ortega, Christina Mendiola, and Gopalrao V. N. Velagaleti -- Detection of chromothripsis in plants / Isabelle M. Henry, Luca Comai, and Ek Han Tan -- RNA-seq analysis to detect abnormal fusion transcripts linked to chromothripsis / Anne-Laure Bougé, Florence Rufflé, Sébastien Riquier, Benoit Guibert, Jérôme Audoux, and Thérèse Commes -- Experimental determination of checkpoint adaptation by mitotic shake-off and microscopy / Lucy H. Swift and Roy M. Golsteyn -- Role for retrotransposons in chromothripsis / Dustin C. Hancks -- Generation of micronuclei and detection of chromosome pulverization / Monique N. H. Luijten, Jeannie X. T. Lee, Sixun Chen, and Karen C. Crasta -- Detection of impaired DNA replication and repair in micronuclei as indicators of genomic instability and chromothripsis / Mariona Terradas, Marta Martín, and Anna Genescà -- Study of telomere dysfunction in TP53 mutant LoVo cell lines as a model for genomicinstability / Oumar Samassekou, Nathalie Bastien, Ju Yan, Sabine Mai, and Régen Drouin -- Genes, proteins, and biological pathways preventing chromothripsis / Martin Poot -- Expression of genes associated with telomere homeostasis in TP53 mutant LoVo cell lines as a model for genomic instability / Oumar Samassekou, Nathalie Bastien, Ju Yan, Sabine Mai, and Régen Drouin -- Chromothripsis detection and characterization using the CTLPScanner web server / Jian Yang, Bo Liu, and Haoyang Cai -- ChromothripsisDB : a curated database for the documentation, visualization, and mining of chromothripsis data / Haoyang Cai -- Time-lapse imaging for the detection of chromosomal abnormalities in primate preimplantation embryos / Brittany L. Daughtry and Shawn L. Chavez -- Correlative live imaging and immunofluorescence for analysis of chromosome segregation in mouse preimplantation embryos / Cayetana Vázquez-Diez and Greg FitzHarris -- Experimental induction of genome chaos / Christine J. Ye, Guo Liu, and Henry H. Heng -- Looking for broken TAD boundaries and changes on DNA interactions : clinical guide to 3D chromatin change analysis in complex chromosomal rearrangements and chromothripsis / Kevin Yauy, Vincent Gatinois, Thomas Guignard, Satish Sati, Jacques Puechberty, Jean Baptiste Gaillard, Anouck Schneider, and Franck Pellestor.
Summary:
This detailed book presents an up-to-date view on methods and experimental approaches developed to identify and explore the chromothripsis phenomenon. Beginning with a section exploring the genesis and impact of chromothripsis, the collection continues by covering the identification of chromothripsis, the causal mechanisms of chromothripsis, the bioinformatics tools for chromothripsis analysis, and experimental systems recently developed for the in vitro investigation of chromothripsis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chromothripsis: Methods and Protocols serves as a vital resource for cell biologists, molecular biologists, cytogeneticists, and geneticists investigating chromothripsis, but also for students and researchers new to the field of chromothripsis and genomic instability.
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.