24. Analysis of plant autophagy / Shi Xiao. Feng Yang, Xiao Tang -- 2. An overview of protein secretion in plant cells / Kin Pau Chung, Yonglun Zeng -- 3. Bioinformatics analysis of protein secretion in plants / Liyuan Chen -- 4. Proteomic analysis of secreted proteins from cell microenvironment / Subash Adhikari, Lan Chen, Peiwu Huang, Ruijun Tian -- 5. Using homology modeling to understand the structural basis of specific interaction of a plant-specific AtSar1a-AtSec23a pair involved in protein ER export / Yap-Shing Nim, Shuangli Sun, Kam-Bo Wong -- 6. Analysis of Golgi-mediated protein traffic in plant cells / Wenjin Shen, Zhidan Xiao, Jinbo Shen, Caiji Gao -- 7. Analysis of membrane protein topology in the plant secretory pathway / Jinya Guo, Yansong Miao, Yi Cai -- 8. Semiautomatic segmentation of plant Golgi stacks in electron tomograms using 3dmod / Keith Ka Ki Mai, Byung-Ho Kang -- 9. 3D printing of plant Golgi stacks from their electron tomographic models / Keith Ka Ki Mai, Madison J. Kang, Byung-Ho Kang -- 10. Transient expression of chimeric fluorescent reporter proteins in pollen tubes to study protein polar secretion and dynamics / Guitao Zhong, Ronghe Liu, Menglong Zhuang, Hao Wang -- 11. Analysis of actin-based intracellular trafficking in pollen tubes / Yuxiang Jiang, Meng Zhang, Shanjin Huang -- 12. Analysis of phragmoplast kinetics during plant cytokinesis / Pantelis Livanos, Mayank Chugh, Sabine Müller -- 13. Immunofluorescence analysis of membrane-associated proteins for calthrin-mediated endocytosis in plant root cells / Chao Wang, Xu Yan, Tingting Meng, Tianwei Hu, Jianwei Pan -- 14. In vivo interaction studies by measuring Förster resonance energy transfer through fluorescence lifetime imaging microscopy (FRET/FLIM) / Florian Fässler, Peter Pimpl -- 15. Analysis of nano-body-epitope interactions in living cells via quantitative protein transport assays / Simone Früholz and Peter Pimpl -- 16. A secretion system for cargo protein identification of vacuolar sorting receptors / Jinbo Shen -- 17. Identifying novel regulators of vacuolar trafficking by combining fluorescence imaging-based forward genetic screening and in vitro pollen germination / Qiang-Nan Feng, Yan Zhang -- 18. Measuring plan protein secretion / Emily R. Larson -- 19. Transient secretory enzyme expression in leaf protoplasts to characterize SNARE functional classes in conventional and unconventional secretion / Di Sansebastiano Gian Pietro, Barozzi Fabrizio -- 20. The organelle pH estimate and measurement in plant secratory pathway / Jinbo Shen -- 21. Analysis of exocyst-positive organelle (EXPO)-mediated unconventional protein secretion (UPS) in plant cells / Yu Ding, Juan Wang -- 22. Isolation of the plant exocyst complex / King Pong Leung, Wilson Chun Yu Lau -- 23. Using microscopy tools to visualize autophagosomal structures in plant cells / Weili Lin, Xiaohong Zhuang -- 24. Analysis of plant autophagy / Liang Chen, Faqiang Li, Shi Xiao.
Series:
Methods in molecular biology, 1064-3745 ; 1662 Springer protocols, 1949-2448
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