Using planar Phi29 pRNA three-way junction to control size and shape of RNA nanoparticles for biodistribution profiling in mice / Farzin Haque, Congcong Xu, Daniel L. Jasinski, Hui Li, and Peixuan Guo. Computational generation of RNA nanorings / Rishabh Sharan, Eckart Bindewald, Wojciech K. Kasprzak, and Bruce A. Shapiro -- Protocols for molecular dynamics simulations of RNA nanostructures / Taejin Kim, Wojciech K. Kasprzak, and Bruce A. Shapiro -- Rolling circle transcription for the self-assembly of multimeric RNAi structures and its applications in nanomedicine / Mihue Jang and Hyung Jun Ahn -- Computational prediction of the immunomodulatory potential of RNA sequences / Gandharva Nagpal, Kumardeep Chaudhary, Sandeep Kumar Dhanda, and Gajendra Pal Singh Raghava -- Cotranscriptional production of chemically modified RNA nanoparticles / Maria L. Kireeva, Kirill A. Afonin, Bruce A. Shapiro, and Mikhail Kashlev -- Supported fluid lipid bilayer as a scaffold to direct assembly of RNA nanostructures / Aleksandra P. Dabkowska, Agnes Michanek, Luc Jaeger, Arkadiusz Chworos, Tommy Nylander, and Emma Sparr -- Evaluation of thermal stability of RNA nanoparticles by temperature gradient gel electrophoresis (TGGE) in native condition / Kheiria Benkato, Benjamin O'Brien, My N. Bui, Daniel L. Jasinski, Peixuan Guo, and Emil F. Khisamutdinov -- Design and crystallography of self-assembling RNA nanostructures / Mark A. Boerneke and Thomas Hermann -- X-aptamer selection and validation / Ganesh L. Lokesh, Hongyu Wang, Curtis H. Lam, Varatharasa Thiviyanathan, Nancy Ward, David G. Gorenstein, and David E. Volk -- Design and preparation of aptamer-siRNA chimeras (AsiCs) for targeted cancer therapy / Sven Kruspe and Paloma H. Giangrande -- Cellular delivery of siRNAs using bolaamphiphiles / Kshitij Gupta -- Preparation and optimization of lipid-like nanoparticles for mRNA Delivery / Bin Li and Yizhou Dong -- Chitosan nanoparticles for miRNA delivery / Merve Denizli, Burcu Aslan, Lingegowda S. Mangala, Dahai Jiang, Cristian Rodriguez-Aguayo, Gabriel Lopez-Berestein, and Anil K. Sood -- Synthesis of PLGA-lipid hybrid nanoparticles for siRNA delivery using the emulsion method PLGA-PEG-lipid nanoparticles for siRNA delivery / Lei Wang, Benjamin Griffel, and Xiaoyang Xu -- Oxime ether lipids as transfection agents : assembly and complexation with siRNA / Anu Puri, Serena Zampino, Mathias Viard, and Bruce A. Shapiro -- Polycationic probe-guided nanopore single-molecule counter for selective miRNA detection / Kai Tian, Ruicheng Shi, Amy Gu, Michael Pennella, and Li-Qun Gu -- Intracellular reassociation of RNA-DNA hybrids that activates RNAi in HIV-infected cells / Angelica N. Martins, Weina Ke, Vaishnavi Jawahar, Morriah Striplin, Caryn Striplin, Eric O. Freed, and Kirill A. Afonin -- Construction and in vivo testing of prokaryotic riboregulators / Alexander A. Green -- Preparation of a conditional RNA switch / Paul Zakrevsky, Lorena Parlea, Mathias Viard, Eckart Bindewald, Kirill A. Afonin, and Bruce A. Shapiro -- Rational engineering of a modular group I ribozyme to control its activity by self-dimerization / Takahiro Tanaka, Yoshiya Ikawa, and Shigeyoshi Matsumura -- CRISPR-Cas RNA scaffolds for transcriptional programming in yeast / Jesse G. Zalatan -- Using planar Phi29 pRNA three-way junction to control size and shape of RNA nanoparticles for biodistribution profiling in mice / Farzin Haque, Congcong Xu, Daniel L. Jasinski, Hui Li, and Peixuan Guo.
Summary:
This volume presents a collection of computational and experimental protocols pertaining to the creation, characterization, and utilization of RNA nanostructures. The chapters in this book cover topics such as ion effects in RNA folding; design and crystallography of self-assembling RNA nanostructures; x-aptamer selection and validation; RNAi in HIV-infected cells; and preparation of a conditional RNA switch. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, RNA Nanostructures: Methods and Protocols is a valuable resource for the design and production of RNA nanostructures. Researchers and scientists sharing these detailed protocols is important for sustained progress in the field.
Series:
Methods in molecular biology, 1064-3745 ; 1632 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.