Conditional modulation of biological processes by low-temperature degrons / Nico Dissmeyer. Apomixis : engineering the ability to harness hybrid vigor in crop plants / Joann A. Conner and Peggy Ozias-Akins -- 3D imaging of whole-mount ovules at cellular resolution to study female germline development in rice / Ethel Mendocilla-Sato, Wenjing She, and Celia Baroux -- Live-cell imaging of F-actin dynamics during fertilization in Arabidopsis thaliana / Daichi Susaki, Daisuke Maruyama, Ramesh Yelagandula, Frederic Berger, and Tomokazu Kawashima -- Development and observation of mature megagametophyte cell-specific fluorescent markers / Mark A. Chamberlin and Shai J. Lawit -- Analysis of fluorescent reporter activity in the male germline during pollen development by confocal microscopy / David Twell and Lynette Brownfield -- In vivo ploidy determination of Arabidopsis thaliana male and female gametophytes / Burcu Nur Keceli, Nico De Storme, and Danny Geelen -- Staining and clearing of arabidopsis reproductive tissue for imaging of fluorescent proteins / Daniel Slane, Patrick Burgel, and Martin Bayer -- Live-cell imaging of auxin and cytokinin signaling in maize female gametophytes / Antony M. Chettoor and Matthew M.S. Evans -- Imaging Ca2+ dynamics in wild-type and NADPH oxidase-deficient mutant pollen tubes with yellow cameleon and confocal laser scanning microscopy / Christina Maria Franck, Jens Westermann, and Aurelien Boisson-Dernier -- Immunolocalization of AGPs and pectins in Quercus suber gametophytic structures / Mario Luıs da Costa, Ana Lucia Lopes, Maria Isabel Amorim, and Sılvia Coimbra -- Optimization of cell spreading and image quality for the study of chromosomes in plant tissues / Laksana Kantama, Erik Wijnker, and Hans de Jong -- Whole mount RNA-FISH on ovules and developing seeds / Andrea Bleckmann and Thomas Dresselhaus -- Analysis of peroxisome biogenesis in pollen by confocal microscopy and transmission electron microscopy / Peng-Fei Jia, Hong-Ju Li, and Wei-Cai Yang -- Transmission electron microscopy (TEM) to study histology of pollen and pollen tubes / Peng-Fei Jia, Hong-Ju Li, and Wei-Cai Yang -- Isolation of Arabidopsis pollen, sperm cells, and vegetative nuclei by fluorescence-activated cell sorting (FACS) / Mario R. Santos, Claudia Bispo, and Jorg D. Becker -- Isolation of rice sperm cells for transcriptional profiling / Scott D. Russell, Daniel S. Jones, Sarah Anderson, Xinkun Wang, Venkatesan Sundaresan, and Xiaoping Gou -- Manual isolation of living cells from the Arabidopsis thaliana female gametophyte by micromanipulation / Maria Englhart, Lucija Soljic, and Stefanie Sprunck -- Isolation and detection of small RNAs from pollen / German Martinez -- Analysis of proteins enriched in rice gamete / Takashi Okamoto -- Phosphoprotein enrichment from tobacco mature pollen crude protein extract / Jan Fıla and David Honys -- Identification of cis-regulatory modules that function in the male germline of flowering plants / Benjamin Peters, Jack Aidley, Murray Cadzow, David Twell, and Lynette Brownfield -- RNA-seq data analysis : from raw data quality control to differential expression analysis / Weihong Qi, Ralph Schlapbach, and Hubert Rehrauer -- RNA-seq data analysis protocol : combining in-house and publicly available data / Marc W. Schmid -- Impedance flow cytometry as a tool to analyze microspore and pollen quality / Iris Heidmann and Marco Di Berardino -- Agrobacterium-mediated Sorghum transformation / Emily Wu and Zuo-Yu Zhao -- Use of the Cas9 orthologs from Streptococcus thermophilus and Staphylococcus aureus for non-homologous end-joining mediated site-specific mutagenesis in Arabidopsis thaliana / Jeannette Steinert, Carla Schmidt, and Holger Puchta -- Transgenic reproductive cell ablation / Shai J. Lawit and Mark A. Chamberlin -- In vivo reporters for protein half-life / Pavel Reichman and Nico Dissmeyer -- Conditional modulation of biological processes by low-temperature degrons / Nico Dissmeyer.
Series:
Methods in molecular biology, 1064-3745 ; v. 1669 Springer protocols, 1949-2448
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