Good manufacturing practice (GMP) translation of advanced cellular therapeutics : lessons for the manufacture of erythrocytes as medicinal products / Neil W.A. McGowan, John D.M. Campbell, and Joanne C. Mountford. Using the Zebrafish as an approach to examine the mechanisms of vertebrate erythropoiesis / Martin D. Kafina and Barry H. Paw -- Mouse models of erythropoiesis and associated diseases / Matthew P. Parker and Kenneth R. Peterson -- Dissecting regulatory mechanisms using mouse fetal liver-derived erythroid cells / Skye C. McIver, Kyle J. Hewitt, Xin Gao, Charu Mehta, Jing Zhang, and Emery H. Bresnick -- Stress erythropoiesis model systems / Laura F. Bennett, Chang Liao, and Robert F. Paulson -- Approaches for analysis of erythroid cell parameters and hemoglobinopathies in mouse models / Marie Trudel and Josepha-Clara Sedzro -- Functional analysis of erythroid progenitors by colony-forming assays / James Palis and Anne Koniski -- Analyzing the formation, morphology, and integrity of erythroblastic islands / Jia Hao Yeo, Matthew P. Cosgriff, and Stuart T. Fraser -- Flow cytometry (FCM) analysis and fluorescence-activated cell sorting (FACS) of erythroid cells / Xiuli An and Lixiang Chen -- Analysis of erythropoiesis using imaging flow cytometry / Theodosia Kalfa and Kathleen E. McGrath -- Flow cytometric analysis of erythroblast enucleation / Xiuli An and Lixiang Chen -- High-resolution fluorescence microscope imaging of erythroblast structure / Alyson Smith, Roberta B. Nowak, and Velia M. Fowler -- Chromatin immunoprecipitation (ChIP) with erythroid samples / Ivan Krivega and Ann Dean -- Chromosome conformation capture (3C and higher) with erythroid samples / Ivan Krivega and Ann Dean -- Genome editing of erythroid cell culture model systems / Jinfen J. Yik, Merlin Crossley, and Kate G.R. Quinlan - In vitro erythroid differentiation and lentiviral knockdown in human CD34+ cells from umbilical cord blood / Anna Kovilakath, Safa Mohamad, Farrah Hermes, Shou Zhen Wang, Gordon D. Ginder, and Joyce A. Lloyd -- Growing and genetically manipulating human umbilical cord blood-derived erythroid progenitor (HUDEP) cell lines / Divya S. Vinjamur and Daniel E. Bauer -- Good manufacturing practice (GMP) translation of advanced cellular therapeutics : lessons for the manufacture of erythrocytes as medicinal products / Neil W.A. McGowan, John D.M. Campbell, and Joanne C. Mountford.
Summary:
This detailed volume provides thorough protocols describing how to use genetics to study mouse and zebrafish erythropoiesis in whole animal models and for genetically manipulating cultured mouse and human erythroid cells.
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.