Quantitative detection of nucleocytoplasmic transport of native proteins in single cells. A microfluidic device for immunoassay-based protein analysis of single E. coli bacteria -- Enzyme-linked immunospot (ELISpot) for single-cell analysis -- Photocleavable DNA barcoding antibodies for multiplexed protein analysis in single cells -- Genome-wide analysis of protein and mRNA copy numbers in single escherichia coli cells with single-molecule sensitivity -- Microfluidic flow cytometry for single-cell protein analysis -- Microfluidic image cytometry for single-cell phenotyping of human pluripotent stem cells -- Characterizing phenotypes and signaling networks of single human cells by mass cytometry -- Multiplexed peptide-MHC tetramer staining with mass cytometry -- Imaging and mapping of tissue constituents at the single-call level using MALDI MSI and quantitative laser scanning cytometry -- SPLIFF: A single-cell method to map protein-protein interactions in time and space -- Microfluidic proximity ligation assay for profiling signaling networks with single-cell resolution -- Dynamics and interactions of individual proteins in the membrane of single, living cells -- Microfluidics-enabled enzyme activity measurement in single cells -- Microfluidic chemical cytometry for enzyme assays of single cells -- Quantitative detection of nucleocytoplasmic transport of native proteins in single cells.
Summary:
This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell level. The book also includes chapters covering a suite of biochemical and biophysical methods capable of making an entire gamut of proteomic measurements, including analysis of protein abundance or expression, protein interaction networks, post-translational modifications, translocation and enzymatic activity.
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.