Delivery of therapeutics to the lung / Dominique N. Price and Pavan Muttil. Overview of innate lung immunity and inflammation / David W. H. Riches and Thomas R. Martin -- Isolation and characterization of mononuclear phagocytes in the mouse lung and lymph nodes / Sophie L. Gibbings and Claudia V. Jakubzick -- Isolation and characterization of mouse neutrophils / Niki D. J. Ubags and Benjamin T. Suratt -- Isolation and characterization of mouse fibroblasts / Benjamin L. Edelman and Elizabeth F. Redente -- Isolation of rat and mouse alveolar type II epithelial cells / Nicole L. Jansing, Jazalle McClendon, Hidenori Kage, Mitsuhiro Sunohara, Juan R. Alvarez, Zea Borok, and Rachel L. Zemans -- Isolation and characterization of human alveolar type II cells / Beata Kosmider, Robert J. Mason, and Karim Bahmed -- Air-liquid interface culture of human and mouse airway epithelial cells / Di Jiang, Niccolette Schaefer, and Hong Wei Chu -- Isolation and characterization of human lung myeloid cells / Yen-Rei A. Yu and Robert M. Tighe -- Measurement of protein permeability and fluid transport of human alveolar epithelial type II cells under pathological conditions / Xiaohui Fang and Michael A. Matthay -- Measuring innate immune function in mouse mononuclear phagocytes / John Matthew Craig and Neil Raj Aggarwal -- Measuring neutrophil bactericidal activity / Kenneth C. Malcolm -- Modulation of myeloid cell function using conditional and inducible transgenic approaches / Alexandra L. McCubbrey and William J. Janssen -- Modulation of lung epithelial cell function using conditional and inducible transgenic approaches / Adrianne L. Stefanski, Dorota S. Raclawska, and Christopher M. Evans -- Computational analysis of RNA-Seq data from airway epithelial cells for studying lung disease / Nathan D. Jackson, Lando Ringel, and Max A. Seibold -- Application of proteomics in lung research / Nichole A. Reisdorph, Cole Michel, Kristofer Fritz, and Richard Reisdorph -- Application of metabolomics in lung Research / Nichole A. Reisdorph, Charmion Cruickshank-Quinn, Yasmeen Nkrumah-Elie, and Richard Reisdorph -- Functional genomics in murine macrophages / Frank Fang-Yao Lee and Scott Alper -- Assessment of ozone-induced lung injury in mice / Vandy P. Stober and Stavros Garantziotis -- Lung histological methods / Aneta Gandjeva and Rubin M. Tuder -- Intravital microscopy in the mouse lung / Yimu Yang, Joseph A. Hippensteel, and Eric P. Schmidt -- Mouse models of acute lung injury and ARDS / Franco R. D'Alessio -- Mouse models of asthma / Magdalena M. Gorska -- Animal models of pulmonary fibrosis / David N. O'Dwyer and Bethany B. Moore -- Mouse models of COPD / Karina A. Serban and Irina Petrache -- Mouse models of viral infection / Kerry M. Empey, R. Stokes Peebles Jr, and William J. Janssen -- Delivery of therapeutics to the lung / Dominique N. Price and Pavan Muttil.
Summary:
This detailed book explores methods to isolate, characterize, and investigate key lung innate immune cells. Beginning with an overview, the volume then continues with methods for creating in vitro and in vivo model systems to study inflammatory lung diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Lung Innate Immunity and Inflammation: Methods and Protocols aims to be a guidebook and will be of value and interest to researchers investigating innate immunity and inflammation in the lung as well as other organs and tissues.
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.