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Title:
Germinal centers : methods and protocols / edited by Dinis Pedro Calado.
Publisher:
Humana Press,
Copyright Date:
2017
Description:
xi, 338 pages : illustrations (some color) ; 26 cm
Subject:
Germinal centers--Laboratory manuals.
Laboratory manuals.
Other Authors:
Calado, Dinis Pedro, editor.
Notes:
Includes bibliographical references and index.
Contents:
How to simulate a germinal center / Philippe A. Robert, Ananya Rastogi, Sebastian C. Binder, and Michael Meyer-Hermann. Detecting gene expression in lymphoid microenvironments by laser microdissection and quantitative RT-PCR / Yang Zhang, Laura Garcia-Ibanez, Geoffrey Brown, and Kai-Michael Toellner -- Tracking plasma cell differentiation in living mice with two-photon microscopy / Carolin Ulbricht, Randall L. Lindquist, Laura Tech, and Anja E. Hauser -- Microanatomical labeling of germinal center structures for flow cytometry using photoactivation / Johanne T. Jacobsen and Gabriel D. Victora -- Fate mapping and transcript profiling of germinal center cells by two-photon photoconversion / Imogen Moran and Tri Giang Phan -- Intravital microscopy of T-B cell interactions in germinal centers / Changming Shih and Hai Qi -- Identifying follicular regulatory T cells by confocal microscopy / Ine Vanderleyden and Michelle A. Linterman -- Cytokine expression by T follicular helper cells / Christoph Jandl, Claudia Loetsch, and Cecile King -- Follicular dendritic cell isolation and loading of immune complexes / Balthasar A. Heesters, Cees E. Van der Poel, and Michael C. Carroll -- Isolation and characterization of mouse and human follicular dendritic cells / Mohey Eldin M. El Shikh, Riham El Sayed, and Costantino Pitzalis -- In vitro-induced germinal center B cell culture system / Kei Haniuda, Takuya Nojima, and Daisuke Kitamura -- CRISPR/Cas9-mediated in vitro mutagenesis in GC-like B cells / Van Trung Chu, Robin Graf, and Klaus Rajewsky -- Germinal center formation with retrovirally transduced B cells for determining the role of specific molecules in vivo / Rinako Nakagawa -- Characterization of the B cell transcriptome bound by RNA-binding proteins with iCLIP / Manuel D. Dıaz-Munoz, Elisa Monzon-Casanova, and Martin Turner -- Flow-cytometric method measuring B cell surface immunoglobulin avidity / Davide Angeletti, Gregory M. Frank, and Jonathan W. Yewdell -- Somatic hypermutation and affinity maturation analysis using the 4-hydroxy-3-nitrophenyl-acetyl (NP) system / Nicole Heise and Ulf Klein -- Targeting gene function in germinal center B cells : a practical approach / Valentina Petrocelli and Stefano Casola -- Development of mouse model systems of germinal center lymphomas / Eleni Kabrani and Sandrine Sander -- AID-Cre-ERT2 model : a tool for monitoring B cell immune responses and generating selective hybridomas / Simon Le Gallou, Takuya Nojima, Daisuke Kitamura, Jean-Claude Weill, and Claude-Agne`s Reynaud -- Determining the origin of human germinal center B cell-derived malignancies / Marc Seifert and Ralf Kuppers -- Tracking B-cell repertoires and clonal histories in normal and malignant lymphocytes / Nicola J. Weston-Bell, Graeme Cowan, and Surinder S. Sahota -- How to simulate a germinal center / Philippe A. Robert, Ananya Rastogi, Sebastian C. Binder, and Michael Meyer-Hermann.
Series:
Methods in molecular biology, 1064-3745 ; volume 1623
Springer protocols, 1949-2448
ISBN:
1493970941
9781493970940
OCLC:
(OCoLC)978289945
LCCN:
2017941511
Locations:
OVUX522 -- University of Iowa Libraries (Iowa City)

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