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05438aam a2200409 i 4500 001 849819B6840911E89478B85797128E48 003 SILO 005 20180710010618 008 171120t20182018nyua b 001 0deng d 010 $a 2018933182 020 $a 9781493976829 020 $a 1493976826 035 $a (OCoLC)1012302002 040 $a YDX $b eng $c YDX $d BDX $d NZHMA $d YDX $d OCLCO $d NUI $d SILO 245 00 $a Viral metagenomics : $b methods and protocols / $c edited by Vitantonio Pantaleo and Michela Chiumenti. 264 1 $a New York, NY : $b Humana Press ; $c [2018] 300 $a xi, 217 pages : $b illustrations (some color) ; $c 26 cm. 490 1 $a Methods in molecular biology, $x 1046-3745 ; $v volume 1746 490 1 $a Springer protocols, $x 1949-2448 504 $a Includes bibliographical references and index. 505 00 $t Insect virus discovery by metagenomic and cell culture-based approaches / $r Finny S. Varghese and Ronald P. van Rij. $t Small RNA isolation from tissues of grapevine and woody plants / $r Annalisa Giampetruzzi, Michela Chiumenti, Angelantonio Minafra, and Pasquale Saldarelli -- $t Double-stranded RNA-enriched preparations to identify viroids by next-generation sequencing / $r Beatriz Navarro and Francesco Di Serio -- $t Viral double-stranded RNAs (dsRNAs) from plants : alternative nucleic acid substrates for high-throughput sequencing / $r Armelle Marais, Chantal Faure, Bernard Bergey, and Thierry Candresse -- $t Workup of human blood samples for deep sequencing of HIV-1 genomes / $r Marion Cornelissen, Astrid Gall, Antoinette van der Kuyl, Chris Wymant, François Blanquart, Christophe Fraser, and Ben Berkhout -- $t Monolith chromatography as sample preparation step in virome studies of water samples / $r Ion GutieÌrrez-Aguirre, Denis Kutnjak, Nejc RacÌki, MatevzÌ Rupar, and Maja Ravnikar -- $t Viral metagenomics approaches for high-resolution screening of multiplexed arthropod and plant viral communities / $r Sarah François, Denis Filloux, Emmanuel Fernandez, MyleÌne Ogliastro, and Philippe Roumagnac -- $t Different approaches to discover mycovirus associated to marine organisms / $r Luca Nerva, Giovanna C. Varese, and Massimo Turina -- $t Use of siRNAs for diagnosis of viruses associated to woody plants in nurseries and stock collections / $r Nikoletta Czotter, JaÌnos MolnaÌr, ReÌka Pesti, Emese DemiaÌn, DaÌniel BaraÌth, TuÌnde Varga, and EÌva VaÌrallyay -- $t Use of high-throughput sequencing for the study and diagnosis of plant viruses and viroids in pollen / $r Kris De Jonghe, Annelies Haegeman, Yoika Foucart, and Martine Maes -- $t High-resolution screening of viral communities and identification of new pathogens in fish using next-generation sequencing / $r Arnfinn Lodden Ãkland, Are Nylund, Ali May, Adalberto Costessi, and Walter Pirovano -- $t Metagenomic analyses of the viruses detected in mycorrhizal fungi and their host orchid / $r Hanako Shimura, Chikara Masuta, and Yasunori Koda -- $t DNA multiple sequence alignment guided by protein domains : the MSA-PAD 2.0 method / $r Bachir Balech, Alfonso Monaco, Michele Perniola, Monica Santamaria, Giacinto Donvito, Saverio Vicario, Giorgio Maggi, and Graziano Pesole -- $t From whole-genome shotgun sequencing to viral community profiling : the ViromeScan tool / $r Simone Rampelli and Silvia Turroni -- $t Shannon entropy to evaluate substitution rate variation among viral nucleotide positions in datasets of viral siRNAs / $r Aysan Ghasemzadeh, Marta MaÅgorzatater Haar, Masoud Shams-bakhsh, Walter Pirovano, and Vitantonio Pantaleo -- $t Insect virus discovery by metagenomic and cell culture-based approaches / $r Finny S. Varghese and Ronald P. van Rij. 520 $a This volume explores the use of viral metagenomics to diagnose known viruses for plant and food production, human and animal health, and identifying viral vectors like insects. The chapters in this book cover topics, such as sRNAs isolation from tissues of grapevines and woody plants, high-resolution screening of arthropod and plant viral communities, identifying new pathogens in fish, detecting viruses in mycorrhizal fungi and their orchid host, and insect virus discovery through metagenomic and cell culture-based approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Viral Metagenomics: Methods and Protocols is a valuable resource for researchers and specialists who are interested in learning more about this evolving field. 650 0 $a Metagenomics $v Laboratory manuals. 650 0 $a Viral genetics $v Laboratory manuals. 650 2 $a Metagenomics. $0 (DNLM)D056186 650 2 $a Viruses $x genetics. $0 (DNLM)D014780Q000235 650 2 $a RNA, Viral. $0 (DNLM)D012367 650 2 $a Plant Viruses $x genetics. $0 (DNLM)D010942Q000235 655 7 $a Laboratory manuals. $2 lcgft 700 1 $a Pantaleo, Vitantonio, $e editor. 700 1 $a Chiumenti, Michela, $e editor. 773 18 $w 990001282000202771 $g no:1746 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 1746, $x 1064-3745 830 0 $a Springer protocols (Series), $x 1949-2448 941 $a 1 952 $l OVUX522 $d 20190212015108.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=849819B6840911E89478B85797128E48Initiate Another SILO Locator Search