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03604aam a2200349 a 4500 001 E3FCC350EE4E11E0BFB3B9EB6AFF544E 003 SILO 005 20111004010313 008 101205s2011 nyua b 001 0 eng d 020 $a 9781617790461 (ebk.) 020 $a 161779046X (ebk.) 020 $a 9781617790454 020 $a 1617790451 035 $a (OCoLC)690089587 040 $a BTCTA $b eng $c BTCTA $d SILO $d RML $d TEF $d HNK $d NUI $d SILO 245 0 $a Argonaute proteins : $b methods and protocols / $c edited by Tom C. Hobman, Thomas F. Duchaine. 260 $a New York : $b Humana Press, $c c2011. 300 $a xi, 344 p. : $b ill. (some col.) ; $c 27 cm. 490 1 $a Methods in molecular biology, $x 1064-3745 ; $v 725 490 1 $a Springer protocols 504 $a Includes bibliographical references and index. 505 00 $t Detection of human dicer and argonaute 2 catalytic activity / $r Marjorie P. Perron ... [et al.] -- $t Chromatin immunoprecipitation in fission yeast / $r Thomas A. Volpe and Jessica DeMaio -- $t Biochemical analyzes of endogenous argonaute complexes immunopurified with anti-argonaute monoclonal antibodies / $r Keita Miyoshi ... [et al.] -- $t Mapping of Ago2-GW182 functional interactions / $r Bing Yao ... [et al.] -- $t Continuous density gradients to study argonaute and GW182 complexes associated with the endocytic pathway / $r Derrick Gibbings -- $t In vitro RISC cleavage assay / $r Julia Stoehr and Gunter Meister -- $t Native gel analysis for RISC assembly / $r Tomoko Kawamata and Yukihide Tomari -- $t Purification and assembly of human argonaute, dicer, and TRBP complexes / $r Nabanita De and Ian J. MacRae -- $t Detection of human dicer and argonaute 2 catalytic activity / $r Marjorie P. Perron ... [et al.] -- 505 80 $t Argonaute pull-down and RISC analysis using 2'-O -methylated oligonucleotides affinity matrices / $r Guillaume Jannot, Alejandro Vasquez-Rifo, and Martin J. Simard -- $t Live Cell imaging of argonaute proteins in mammalian cells / $r Justin M. Pare, Joaquin Lopez-Orozco, and Tom C. Hobman -- $t Reporter-based assays for analyzing RNA interference in mammalian cells / $r Lydia V. McClure, Gil Ju Seo, and Christopher S. Sullivan -- $t Artificial tethering of argonaute proteins for studying their role in translational repression of target mRNAs / $r Stephanie Eckhardt ... [et al.] -- $t Efficient system for Let-7 microRNA and GW182 protein-mediated deadenylation in vitro / $r Marc R. Fabian, Yuri V. Svitkin, and Nahum Sonenberg -- $t Cell-free microRNA-mediated translation repression in Caenorhabditis elegans / $r Edlyn Wu and Thomas F. Duchaine -- $t Argonaute pull-down and RISC analysis using 2'-O -methylated oligonucleotides affinity matrices / $r Guillaume Jannot, Alejandro Vasquez-Rifo, and Martin J. Simard -- 505 80 $t Whole cell proteome regulation by microRNAs captured in a pulsed SILAC mass spectrometry approach / $r Olivia A. Ebner and Matthias Selbach. $t Immunoprecipitation of piRNPs and directional, next generation sequencing of piRNAs / $r Yohei Kirino ... [et al.] -- $t Generation of an inducible mouse ES cell lines deficient for argonaute proteins / $r Hong Su and Xiaozhong Wang -- $t Whole cell proteome regulation by microRNAs captured in a pulsed SILAC mass spectrometry approach / $r Olivia A. Ebner and Matthias Selbach. 650 0 $a RNA. 700 1 $a Hobman, Tom C., $d 1961- 700 1 $a Duchaine, Thomas, $d 1974- 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 725. 830 0 $a Springer protocols. 941 $a 1 952 $l OVUX522 $d 20180109021050.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=E3FCC350EE4E11E0BFB3B9EB6AFF544EInitiate Another SILO Locator Search