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04688aam a2200385 a 4500 001 CD36A3EC019E11E89C78220097128E48 003 SILO 005 20180125010234 008 120314t20122012nyua b 001 0 eng d 010 $a 2012938221 020 $a 1617799300 020 $a 9781617799303 035 $a (OCoLC)780478165 040 $a BTCTA $b eng $c BTCTA $d RML $d EYM $d OCLCO $d YDXCP $d BWX $d COU $d CDX $d NUI $d UtOrBLW $d SILO 050 4 $a QH506 $b .M45 v.901 245 00 $a Antibody methods and protocols / $c edited by Gabriele Proetzel, Hilmar Ebesbach. 264 1 $a New York : $b Humana Press : $c [2012] 300 $a x, 325 pages : $b illustrations ; $c 27 cm. 490 1 $a Methods in molecular biology, $x 1064-3745 ; $v 901 490 1 $a Springer protocols 504 $a Includes bibliographical references and index. 505 00 $t Class-specific effector functions of therapeutic antibodies / $r Virginie Pascal, Brice Laffleur, and Michel CogneÌ. $t Recombinant antibodies and in vitro selection technologies / $r C. Ronald Geyer ... [et al.] -- $t Phage display / $r Konstantin Petropoulos -- $t Generation of human fab libraries for phage display / $r Christoph Rader -- $t Selection of human fab libraries by phage display / $r Christoph Rader -- $t Ribosome Display / $r George Thom and Maria Groves -- $t Hybridoma technology for the generation of monoclonal antibodies / $r Chonghui Zhang -- $t Application of transgenic mice for therapeutic antibody discovery / $r E-Chiang Lee and Michael Owen -- $t Production of human or humanized antibodies in mice / $r Brice Laffleur ... [et al.] -- $t Immortalization of human B cells : analysis of B cell repertoire and production of human monoclonal antibodies / $r Elisabetta Traggiai -- $t Kinetic screening in the antibody development process / $r Michael SchraÌml and Matthias Biehl -- $t Temperature-dependent antibody kinetics as a tool in antibody lead selection / $r Michael SchraÌml and Leopold von Proff -- $t Determination of antibody glycosylation by mass spectrometry / $r Christiane JaÌger ... [ et al.] -- $t Cloning, expression, and purification of monoclonal antibodies in scFv-Fc format / $r Jiahui Yang and Christoph Rader -- $t PEGylation of antibody fragments for half-life extension / $r Simona JevsÌevar, Mateja Kusterle, and Maja Kenig -- $t Bispecific antibody derivatives based on full-length IgG formats / $r Michael Grote ... [et al.] -- $t Generation of fluorescent IgG fusion proteins in mammalian Cells / $r Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann -- $t Methods to engineer and identify IgGâ variants with improved FcRn binding or effector function / $r Robert F. Kelley and Y. Gloria Meng -- $t Class-specific effector functions of therapeutic antibodies / $r Virginie Pascal, Brice Laffleur, and Michel CogneÌ. 520 $a "The rapidly growing field of antibody research is the result of many advancing technologies allowing current developments to take advantage of molecular engineering to create tailor-made antibodies. Antibody Methods and Protocols attempts to provide insight into the generation of antibodies using in vitro and in vivo approaches, as well as technical aspects for screening, analysis, and modification of antibodies and antibody fragments. The detailed volume is focused on basic protocols for isolating antibodies and, at the same time, it selects a range of specific areas with the aim of providing guides for the overall process of antibody isolation and characterization as well as protocols for enhancing classical antibodies and antibody fragments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls"--Publisher. 650 12 $a Antibodies. $2 [MeSH] $0 https://id.nlm.nih.gov/mesh/D000906 650 22 $a Protein Engineering $x methods. $2 [MeSH] $0 https://id.nlm.nih.gov/mesh/D015202Q000379 655 2 $a Laboratory Manuals. 650 0 $a Immunoglobulins. $0 http://id.loc.gov/authorities/subjects/sh85064568 700 1 $a Proetzel, Gabriele, $e editor. $0 http://id.loc.gov/authorities/names/nb2010006026 700 1 $a Ebersbach, Hilmar, $e editor. $0 http://id.loc.gov/authorities/names/nb2012019813 773 18 $w 990001282000202771 $g no:901 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 901. $0 http://id.loc.gov/authorities/names/n92002874 830 0 $a Springer protocols (Series) $0 http://id.loc.gov/authorities/names/no2008180772 941 $a 1 952 $l OVUX522 $d 20180125053658.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=CD36A3EC019E11E89C78220097128E48Initiate Another SILO Locator Search