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05820aam a2200409 i 4500 001 ABC24640580511E8A8F83C5097128E48 003 SILO 005 20180515010114 008 171013t20182018nyua b 001 0deng d 010 $a 2017963090 020 $a 9781493975945 020 $a 1493975943 035 $a (OCoLC)1006301974 040 $a YDX $b eng $c YDX $d NUI $d SILO 245 00 $a Proteases and cancer : $b methods and protocols / $c edited by Santiago Cal and Alvaro J. Obaya. 264 1 $a New York, N.Y. : $b Humana Press : $c [2018] 300 $a xv, 352 pages : $b illustrations (some color) ; $c 26 cm. 490 1 $a Methods in molecular biology, $x 1064-3745 ; $v 1731 490 1 $a Springer protocols, $x 1949-2448 504 $a Includes bibliographical references and index. 505 00 $t Strategies to target matrix metalloproteinases as therapeutic approach in cancer / $r Zoi Piperigkou, Dimitra Manou, Konstantina Karamanou, and Achilleas D. Theocharis. $t Identification of protease cleavage sites and substrates in cancer by Carboxy-TAILS (C-TAILS) / $r Nestor Solis and Christopher M. Overall -- $t Identification of proteolytic cleavage sites of EphA2 by membrane type 1 matrix metalloproteinase on the surface of cancer cells/ $r Keiji Kikuchi, Hiroko Kozuka-Hata, Masaaki Oyama, Motoharu Seiki, and Naohiko Koshikawa -- $t Biotin-chasing assay to evaluate uPAR stability and cleavage on the surface of cells / $r Vladimir Leksa, Herbert B. Schiller, and Hannes Stockinger -- $t Determination of aconitase activity : a substrate of the mitochondrial lon protease / $r Pedro M. QuiroÌs -- $t Simple cell-based assay for the detection of surface protein shedding by rhomboid proteases / $r Angela Moncada-Pazos and Adam Graham Grieve -- $t Functional production of catalytic domains of human MMPs in Escherichia coli periplasm / $r Dong Hyun Nam, Ki Baek Lee, and Xin Ge -- $t Autophagy and proteases : basic study of the autophagic flux by western blot / $r AÌlvaro F. FernaÌndez -- $t Gel-based gelatin zymography to examine matrix metalloproteinase activity in cell culture / $r Aastha Chhabra and Vibha Rani -- $t Analysis of enzymatic activity of matrix metalloproteinase (MMP) by collagen zymography in melanoma / $r Vijay Walia and Yardena Samuels -- $t Measurement of protease activities using fluorogenic substrates/ $r Salvatore Santamaria and Hideaki Nagase -- $t Targeting the expression of cathepsin B using CRISPR/Cas9 system in mammalian cancer cells / $r Manu Gnanamony and Christopher S. Gondi -- $t Assessing the influence of a protease in cell migration using the barrier-migration assay / $r Tania Fontanil, Yamina Mohamedi, Santiago Cal, and AÌlvaro J. Obaya -- $t Analysis of invasive activity of CAF spheroids into three dimensional (3D) collagen matrices / $r MariÌa AÌngeles Villaronga, SauÌl AÌlvarez Teijeiro, Francisco Hermida-Prado, Marta GarzoÌn-Arango, Victoria Sanz-Moreno, and Juana MariÌa GarciÌa-Pedrero -- $t Beyond the proteolytic activity : examining the functional relevance of the ancillary domains using tri-dimensional (3D) spheroid invasion assay / $r Erico Tosoni Costa and Anamaria Aranha Camargo -- $t Analyzing the type II transmembrane serine protease hepsin-dependent basement membrane remodeling in 3D cell culture / $r Shishir M. Pant, Denis Belitskin, Hanna Ala-Hongisto, Juha KlefstroÌm, and Topi A. Tervonen -- $t Evaluation of tumor vasculature using a syngeneic tumor model in wild-type and genetically modified mice / $r Francisco Javier RodriÌguez-Baena, Silvia Redondo-GarciÌa, MariÌa del Carmen Plaza-Calonge, RubeÌn FernaÌndez-RodriÌguez, and Juan Carlos RodriÌguez-Manzaneque -- $t 3D image analysis of the microvasculature in healthy and diseased tissues / $r AÌlvaro SahuÌn-EspanÌol, Cristina Clemente, and Alicia G. Arroyo -- $t Human tumor tissue-based 3D in vitro invasion assays / $r Pirjo AÌstroÌm, Ritva Heljasvaara, Pia Nyberg, Ahmed Al-Samadi, and Tuula Salo -- $t Ear sponge assay : a method to investigate angiogenesis and lymphangiogenesis in mice / $r Maureen Van de Velde, Melissa GarciÌa-Caballero, Tania DurreÌ, FreÌdeÌric Kridelka, and AgneÌs NoeÌl -- $t Cancer susceptibility models in protease-deficient mice / $r Alicia R. Folgueras, Sandra Freitas-RodriÌguez, Yaiza EspanÌol, and Gloria Velasco -- $t Imaging proteolytic activities in mouse models of cancer / $r Anupama Pal and Alnawaz Rehemtulla -- $t Protease silencing to explore the molecular mechanisms of cancer and aging / $r Julia M. Fraile, Diana Campos-Iglesias, and JoseÌ M.P. Freije -- $t Analysis of somatic DNA methylation alterations of genes encoding cell surface metallopeptidases in colorectal cancer / $r Sergio Alonso, Beatriz GonzaÌlez, Andreu AlibeÌs, and Manuel Perucho -- $t Targeting deubiquitinases in cancer / $r Joseph S. Bednash and Rama K. Mallampalli -- $t Generation of highly selective MMP antibody inhibitors / $r Dong Hyun Nam and Xin Ge -- $t Strategies to target matrix metalloproteinases as therapeutic approach in cancer / $r Zoi Piperigkou, Dimitra Manou, Konstantina Karamanou, and Achilleas D. Theocharis. 650 0 $a Proteolytic enzymes $v Laboratory manuals. 650 0 $a Cancer $x Etiology $v Laboratory manuals. 650 7 $a Proteolytic enzymes. $2 fast $0 (OCoLC)fst01079783 650 7 $a Cancer $x Etiology. $2 fast $0 (OCoLC)fst00845361 650 2 $a Neoplasms. 650 2 $a Peptide Hydrolases. 655 7 $a Laboratory manuals. $2 lcgft 655 7 $a Laboratory manuals. $2 fast $0 (OCoLC)fst01920776 700 1 $a Cal, Santiago, $e editor. 700 1 $a Obaya, Alvaro J., $e editor. 773 18 $w 990001282000202771 $g v:1731 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 1731. $x 1064-3745 830 0 $a Springer protocols (Series), $x 1949-2448 941 $a 1 952 $l OVUX522 $d 20181116021031.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=ABC24640580511E8A8F83C5097128E48Initiate Another SILO Locator Search