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05412aam a2200433 i 4500 001 FAD59C9CE96D11E8978F920F97128E48 003 SILO 005 20181116010210 008 180317t20182018nyua b 001 0deng d 010 $a 2018946775 020 $a 9781493985692 020 $a 1493985698 035 $a (OCoLC)1029206592 040 $a YDX $b eng $c YDX $d NUI $d SILO 245 00 $a Lung innate immunity and inflammation : $b methods and protocols / $c edited by Scott Alper, William J. Janssen. 264 1 $a New York : $b Humana Press ; $c [2018] 300 $a xii, 433 pages : $b illustrations (some color) ; $c 25 cm 490 1 $a Methods in molecular biology, $x 1064-3745 ; $v volume 1809 490 1 $a Springer protocols, $x 1949-2448 504 $a Includes bibliographical references and index. 505 00 $t Delivery of therapeutics to the lung / $r Dominique N. Price and Pavan Muttil. $t Overview of innate lung immunity and inflammation / $r David W. H. Riches and Thomas R. Martin -- $t Isolation and characterization of mononuclear phagocytes in the mouse lung and lymph nodes / $r Sophie L. Gibbings and Claudia V. Jakubzick -- $t Isolation and characterization of mouse neutrophils / $r Niki D. J. Ubags and Benjamin T. Suratt -- $t Isolation and characterization of mouse fibroblasts / $r Benjamin L. Edelman and Elizabeth F. Redente -- $t Isolation of rat and mouse alveolar type II epithelial cells / $r Nicole L. Jansing, Jazalle McClendon, Hidenori Kage, Mitsuhiro Sunohara, Juan R. Alvarez, Zea Borok, and Rachel L. Zemans -- $t Isolation and characterization of human alveolar type II cells / $r Beata Kosmider, Robert J. Mason, and Karim Bahmed -- $t Air-liquid interface culture of human and mouse airway epithelial cells / $r Di Jiang, Niccolette Schaefer, and Hong Wei Chu -- $t Isolation and characterization of human lung myeloid cells / $r Yen-Rei A. Yu and Robert M. Tighe -- $t Measurement of protein permeability and fluid transport of human alveolar epithelial type II cells under pathological conditions / $r Xiaohui Fang and Michael A. Matthay -- $t Measuring innate immune function in mouse mononuclear phagocytes / $r John Matthew Craig and Neil Raj Aggarwal -- $t Measuring neutrophil bactericidal activity / $r Kenneth C. Malcolm -- $t Modulation of myeloid cell function using conditional and inducible transgenic approaches / $r Alexandra L. McCubbrey and William J. Janssen -- $t Modulation of lung epithelial cell function using conditional and inducible transgenic approaches / $r Adrianne L. Stefanski, Dorota S. Raclawska, and Christopher M. Evans -- $t Computational analysis of RNA-Seq data from airway epithelial cells for studying lung disease / $r Nathan D. Jackson, Lando Ringel, and Max A. Seibold -- $t Application of proteomics in lung research / $r Nichole A. Reisdorph, Cole Michel, Kristofer Fritz, and Richard Reisdorph -- $t Application of metabolomics in lung Research / $r Nichole A. Reisdorph, Charmion Cruickshank-Quinn, Yasmeen Nkrumah-Elie, and Richard Reisdorph -- $t Functional genomics in murine macrophages / $r Frank Fang-Yao Lee and Scott Alper -- $t Assessment of ozone-induced lung injury in mice / $r Vandy P. Stober and Stavros Garantziotis -- $t Lung histological methods / $r Aneta Gandjeva and Rubin M. Tuder -- $t Intravital microscopy in the mouse lung / $r Yimu Yang, Joseph A. Hippensteel, and Eric P. Schmidt -- $t Mouse models of acute lung injury and ARDS / $r Franco R. D'Alessio -- $t Mouse models of asthma / $r Magdalena M. Gorska -- $t Animal models of pulmonary fibrosis / $r David N. O'Dwyer and Bethany B. Moore -- $t Mouse models of COPD / $r Karina A. Serban and Irina Petrache -- $t Mouse models of viral infection / $r Kerry M. Empey, R. Stokes Peebles Jr, and William J. Janssen -- $t Delivery of therapeutics to the lung / $r Dominique N. Price and Pavan Muttil. 520 8 $a This detailed book explores methods to isolate, characterize, and investigate key lung innate immune cells. Beginning with an overview, the volume then continues with methods for creating in vitro and in vivo model systems to study inflammatory lung diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Lung Innate Immunity and Inflammation: Methods and Protocols aims to be a guidebook and will be of value and interest to researchers investigating innate immunity and inflammation in the lung as well as other organs and tissues. 650 0 $a Natural immunity $v Laboratory manuals. 650 0 $a Pneumonia $v Laboratory manuals. 650 7 $a Natural immunity. $2 fast $0 (OCoLC)fst01034361 650 7 $a Pneumonia. $2 fast $0 (OCoLC)fst01067563 650 2 $a Immunity, Innate. 650 12 $a Lung $x immunology. 650 2 $a Inflammation. 655 7 $a Laboratory manuals. $2 fast $0 (OCoLC)fst01920776 655 7 $a Laboratory manuals. $2 lcgft 700 1 $a Alper, Scott, $e editor. 700 1 $a Janssen, William J., $e editor. 773 18 $w 990001282000202771 $g no:31858069604621 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 1809. $x 1064-3745 830 0 $a Springer protocols (Series), $x 1949-2448 941 $a 1 952 $l OVUX522 $d 20190212025552.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=FAD59C9CE96D11E8978F920F97128E48Initiate Another SILO Locator Search