0 Product development and commercialization of diagnostic or life science products for scientists and researchers / Meghan M. Alonso. Innovations in clinical trial design in the era of molecular profiling / Julia D. Wulfkuhle, Alexander Spira, Kirsten H. Edmiston, and Emanuel F. Petricoin III -- Personalized medicine : ethical aspects / G. Terry Sharrer -- Antibody validation by Western Blotting / Michele Signore, Valeria Manganelli, and Alex Hodge -- Scanning electron microscopy sample preparation and imaging / Jenny Ngoc Tran Nguyen and Amanda M. Harbison -- One-step preservation and decalcification of bony tissue for molecular profiling / Claudius Mueller, Michael G. Harpole, and Virginia Espina -- Application of hydrogel nanoparticles for the capture, concentration, and preservation of low-abundance biomarkers / Ruben Magni and Alessandra Luchini -- Using laser capture microdissection to isolate cortical laminae in nonhuman primate brain / Brian A. Corgiat and Claudius Mueller -- Western blot techniques / Brianna Kim -- ELISA for monitoring nerve growth factor / Justin B. Davis -- Reverse phase protein microarrays / Elisa Baldelli, Valerie Calvert, Alex Hodge, Amy VanMeter, Emanuel F. Petricoin III, and Mariaelena Pierobon -- Clustering and network analysis of reverse phase protein array data / Adam Byron -- PCR : identification of genetic polymorphisms / Amanda M. Harbison and Jenny Ngoc Tran Nguyen -- Microsatellite analysis for identification of individuals using bone from the extinct steller's sea cow (Hydrodamalis gigas) / Jeffery F. Warner, Michael G. Harpole, and Lorelei D. Crerar -- Somatic DNA mutation analysis / Anthony O'Grady and Robert Cummins -- Optimization of immunostaining for prospective image analysis / Scott M. Lawrence and Yelena G. Golubeva -- Fluorescence in situ hybridization of cells, chromosomes, and formalin-fixed paraffin-embedded tissues / Ahmad Alamri, Jun Yeb Nam, and Jan K. Blancato -- High-resolution image stitching as a tool to assess tissue-level protein distribution and localization / Bryan A. Millis and Matthew J. Tyska -- Mass spectrometry-based biomarker discovery / Weidong Zhou, Emanuel F. Petricoin III, and Caterina Longo -- Quantitative mass spectrometry by isotope dilution and multiple reaction monitoring (MRM) / Paul Russo, Brian L. Hood, Nicholas W. Bateman, and Thomas P. Conrads -- LC-mass spectrometry for metabolomics / Allyson L. Dailey -- Metabolomic Bioinformatic Analysis / Allyson L. Dailey -- Stable isotope quantitative N-glycan analysis by liquid separation techniques and mass spectrometry / Stefan Mittermayr, Simone Albrecht, Csaba Váradi, Silvia Millán-Martín, and Jonathan Bones -- Grant writing tips for translational research / Lindsay Wescott, Michael Laskofski, Donna Senator, and Carly Curran -- Inventions and patents : a practical tutorial / Hina Mehta, Lille Tidwell, and Lance A. Liotta -- Product development and commercialization of diagnostic or life science products for scientists and researchers / Meghan M. Alonso.
Summary:
This second edition provides an update on technology and an accelerated tutorial to assist, students, entrepreneurs, new investigators, and established investigators who want to quickly become versed in, and immersed in, the entire process from discovery to clinical trial validation and commercial public benefit. Chapters aim to cover the full spectrum of molecular profiling from tumor staging and grading through biomarker discovery, to commercialization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Molecular Profiling: Methods and Protocols, Second Edition aims to be a useful guide on molecular profiling.
Series:
Methods in molecular biology, 1064-3745 ; 1606 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.