Culturing and cryobanking human neural stem cells / Jeremy M. Crook and Eva Tomaskovic-Crook. Quality assurance in stem cell banking : emphasis on embryonic and induced pluripotent stem cell banking / Therése Kallur, Pontus Blomberg, Sonya Stenfelt, Kristian Tryggvason, and Outi Hovatta -- Acquisition and reception of primary tissues, cells, or other biological specimens / Lyn E. Healy -- Information management / Alberto Labarga, Izaskun Beloqui, and Angel G. Martin -- Cryopreservation : vitrification and controlled rate cooling / Charles J. Hunt -- Quality assured characterization of stem cells for safety in banking for clinical application / Kevin W. Bruce, John D.M. Campbell, and Paul De Sousa -- Ethics and governance of stem cell banks / Donald Chalmers, Peter Rathjen, Joy Rathjen, and Dianne Nicol -- Derivation of human Embryonic Stem Cells / Jeremy M. Crook, Lucy Kravets, Teija Peura, and Meri T. Firpo -- Derivation of human-induced pluripotent stem cells in chemically defined medium / Guokai Chen and Mahendra Rao -- Culture, adaptation, and expansion of pluripotent stem cells / Jennifer L. Brehm and Tenneille E. Ludwig -- Cryobanking pluripotent stem cells / Jeremy M. Crook, Eva Tomaskovic-Crook, and Tenneille E. Ludwig -- Genome editing in human pluripotent stem cells / Jared Carlson-Stevermer and Krishanu Saha -- Isolation, culture, and expansion of mesenchymal stem cells / Izaskun Ferrin, Izaskun Beloqui, Lorea Zabaleta, Juan M. Salcedo, Cesar Trigueros, and Angel G. Martin -- Cryobanking mesenchymal stem cells / Andrés Pavón, Izaskun Beloqui, Juan M. Salcedo, and Angel G. Martin -- Culturing and cryobanking human neural stem cells / Jeremy M. Crook and Eva Tomaskovic-Crook.
Summary:
This volume covers protocols related to both pluripotent and somatic stem cells, including the ethical procurement of tissues and cells for the provision of ?seed stock,? standardized methods for deriving hESCs and iPSCs, isolating mesenchymal stem cells, cell culture and cryopreservation, in addition to quality assurance and information management. Stem Cell Banking: Concepts and Protocols aims to contribute to the development of this field by providing information that is essential to establishing a bona fide stem cell bank. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Stem Cell Banking: Concepts and Protocols is a valuable resource for stem cell scientists and novices to the field, and will help strengthen and maximize their use of existing and future stem cell resources.
Series:
Methods in molecular biology, 1064-3745 ; 1590 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.