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Title:
PCR detection of microbial pathogens / edited by Mark Wilks.
Edition:
2nd ed.
Publisher:
Humana Press,
Copyright Date:
c2013
Description:
xi, 317 p. : ill. (some col.) ; 27 cm.
Subject:
Microbiological Techniques--Laboratory Manuals.
Polymerase Chain Reaction--methods--Laboratory Manuals.
Diagnostic Techniques and Procedures--Laboratory Manuals.
Diagnostic microbiology.
Polymerase chain reaction--Diagnostic use.
Molecular diagnosis.
Other Authors:
Wilks, Mark (Microbiologist)
Notes:
Includes bibliographical references and index.
Contents:
Real-time quantitative PCR, pathogen detection and MIQE / Gemma Johnson, Tania Nolan, and Stephen A. Bustin -- Overcoming inhibition in real-time diagnostic PCR / Johannes Hedman and Peter Rådström -- Quality in the molecular microbiology laboratory / Paul S. Wallace and William G. MacKay -- Pre-analytical sample treatment and DNA extraction protocols for the detection of bacterial pathogens from whole blood / Wendy L.J. Hansen, Cathrien A. Bruggeman, and Petra F.G. Wolffs -- Detection of bacterial contamination in platelet concentrates using flow cytometry and real-time PCR methods / Tanja Vollmer, Knut Kleesiek, and Jens Dreier -- Multiplex real-time PCR assay for the detection of meticillin-resistant Staphylococcus aureus and Panton-Valentine leukocidin from clinical samples / Lynne Renwick, Anne Holmes, and Kate Templeton -- PCR Detection of Haemophilus influenzae from respiratory specimens / Guma M.K. Abdeldaim and Björn Herrmann --
Rapid detection of atypical respiratory bacterial pathogens by real-time PCR / Clare L. Ling and Timothy D. McHugh -- Utilization of multiple real-time PCR assays for the diagnosis of Bordetella spp. in clinical specimens / Kathleen M. Tatti and Maria Lucia Tondella -- Detection of Mycoplasma pneumoniae by real-Time PCR / Jonas M. Winchell and Stephanie L. Mitchell -- Real-time PCR assay for the diagnosis of Pneumocystis jirovecii pneumonia / Judith Fillaux and Antoine Berry -- Rapid identification of mycobacteria and rapid detection of drug resistance in Mycobacterium tuberculosis in cultured isolates and in respiratory specimens / Wing-Cheong Yam and Kit-Hang Gilman Siu -- Direct detection of Mycobacterium ulcerans in clinical specimens and environmental samples / Caroline J. Lavender and Janet A.M. Fyfe -- Detection of Bartonella spp. DNA in clinical specimens using an internally controlled real-time PCR assay / Anneke M.C. Bergmans and John W.A. Rossen --
Simultaneous direct identification of genital microorganisms in voided urine using multiplex PCR-based reverse line blot assays / Michelle L. McKechnie, Fanrong Kong, and Gwendolyn L. Gilbert -- Diagnosis of Clostridium difficile infection using real-time PCR / Renate Johanna van den Berg, Dennis Bakker, and Ed J. Kuijper -- Detection of pathogenic Leptospira spp. through real-time PCR (qPCR) targeting the LipL32 gene / Robyn Anne Stoddard -- Sensitive and rapid detection of Campylobacter jejuni and Campylobacter coli using loop-mediated isothermal amplification / Wataru Yamazaki -- PCR detection of Helicobacter pylori in clinical samples / Emiko Rimbara, Masanori Sasatsu, and David Y. Graham -- Rapid detection of the Escherichia coli genospecies in water by conventional and real-time PCR / Andrée F. Maheux, Luc Bissonnette, and Michel G. Bergeron -- Multiplex real-time PCR (MRT-PCR) for diarrheagenic / Francesca Barletta, Theresa J. Ochoa, and Thomas G. Cleary.
Series:
Methods in molecular biology, 1064-3745 ; 943
Springer protocols
ISBN:
1603273530 (eBook)
9781603273534 (eBook)
1603273522 (alk. paper)
9781603273527 (alk. paper)
OCLC:
(OCoLC)820791181
LCCN:
2012948978
Locations:
OVUX522 -- University of Iowa Libraries (Iowa City)

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