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Title:
Cytotoxic T-cells : methods and protocols / edited by Elena Ranieri.
Publisher:
Humana Press,
Copyright Date:
2014
Description:
xii, 301 pages : illustrations (some color) ; 27 cm
Subject:
T cells--Laboratory manuals.
Systems biology--Laboratory manuals.
T-Lymphocytes, Cytotoxic--Laboratory Manuals.
Systems Biology--methods--Laboartory Manuals.
Laboartory Manuals.
Other Authors:
Ranieri, Elena, editior.
Springer Science+Business Media, copyright holder.
Notes:
Includes bibliographical references and index.
Contents:
CTL and transplantation : tissue in vivo characterization / Federica Rascio, Chiara Divella, and Giuseppe Grandaliano. In vitro\ex vivo generation of cytotoxic T lymphocytes / Margherita Gigante and Elena Ranieri -- Phenotypic and functional characterization of cytotoxic T lymphocytes by flow cytometry / Iulia Popescu [and three others] -- Intracellular signaling of CTLs / Sharon Natasha Cox -- Monitoring antigen-specific T cell responses using real-time PCR / Devin B. Lowe, Jennifer L. Taylor, and Walter J. Storkus -- CTL ELISPOT assay / Elena Ranieri, Iulia Popescu, and Margherita Gigante -- Methods of purification of CTL-derived exosomes / Angela Montecalvo, Adriana T. Larregina, and Adrian E. Morelli -- Characterization of CTL by microscopy / Giuseppe Stefano Netti -- Intravital imaging of cytotoxic T lymphocytes / Gaetano Faleo, Per-Olof Berggren, and Antonello Pileggi -- The microarray-based approach for the analysis of the transcriptome / Matteo Accetturo, Paola Pontrelli, and Loreto Gesualdo -- miRNome analysis using real-time PCR / Paola Pontrelli, Matteo Accetturo, and Loreto Gesualdo -- Proteomic approaches by SELDI and MALDI-TOF/MS for CTL analysis / Massimo Papale and Maria Teresa Rocchetti -- Two-dimensional gel electrophoresis approach for CTL phosphoproteome analysis / Maria Teresa Rocchetti, Massimo Papale, and Loreto Gesualdo -- Targeting the JAK/STAT pathway in cytotoxic T lymphocytes (CTL) by next generation sequencing (NGS) / Maddalena Gigante, Sterpeta Diella, and Elena Ranieri -- Experimental model for studying the involvement of regulatory cytotoxic T cells in bone resorption / Giacomina Brunetti [and three others] -- CTL and transplantation : tissue in vivo characterization / Federica Rascio, Chiara Divella, and Giuseppe Grandaliano.
Series:
Methods in molecular biology, 1064-3745 ; 1186
Springer protocols, 1949-2448
ISBN:
1493911570
9781493911578
OCLC:
(OCoLC)879245643
LCCN:
2014944379
Locations:
OVUX522 -- University of Iowa Libraries (Iowa City)

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