Analysis of HIV-1 Gag-RNA interactions in cells and virions by CLIP-seq / Jason W. Rausch [and others] -- Measuring T cell-to-T cell HIV-1 transfer, viral fusion, and infection using flow cytometry / Natasha D. Durham and Benjamin K. Chen -- HIV-1 capsid stabilization assay / Thomas Fricke and Felipe Diaz-Griffero -- Detection and tracking of dual-labeled HIV particles using wide-field live cell imaging to follow viral core Integrity / João I. Mamede and Thomas J. Hope -- HIV-1 reverse transcriptase-based assay to determine cellular dNTP concentrations / Joseph A. Hollenbaugh and Baek Kim -- Rapid eetermination of HIV-1 mutant frequencies and mutation spectra using an mCherry/EGFP dual-reporter viral vector / Jonathan M.O. Rawson, Christine L. Clouser, and Louis M. Mansky -- Novel biochemical tools for probing HIV RNA structure / Jason W. Rausch [and others] -- Analysis of HIV-1 Gag-RNA interactions in cells and virions by CLIP-seq / Milena Veselinovic, Paige Charlins, and Ramesh Akkina -- Modeling HIV-1 mucosal transmission and prevention in humanized mice / Deepali Singh [and others] -- Methods for the analyses of inhibitor-induced aberrant multimerization of HIV-1 integrase / Jacques J. Kessl, Amit Sharma, and Mamuka Kvaratskhelia -- Quantification of HIV-1 gag localization within virus producer cells / Annalena La Porte and Ganjam V. Kalpana -- Methods to study determinants for membrane targeting of HIV-1 gag in vitro / Gabrielle C. Todd and Akira Ono -- Visualizing the behavior of HIV-infected T cells in vivo using multiphoton intravital microscopy / Radwa Sharaf, Thorsten R. Mempel, and Thomas T. Murooka -- Modeling HIV-1 mucosal transmission and prevention in humanized mice / Milena Veselinovic, Paige Charlins, and Ramesh Akkina -- Detecting HIV-1 Tat in cell culture supernatants by ELISA or western blot / Fabienne Rayne [and others] -- Measuring the frequency of latent HIV-1 in resting CD4+ T cells using a limiting dilution Coculture Assay / Gregory M. Laird [and others] -- LGIT in vitro latency model in primary and T cell lines to test HIV-1 reactivation compounds / Ulrike Jung [and others] -- Improved methods to detect low levels of HIV using antibody-based technologies / Eliseo A. Eugenin and Joan W. Berman -- Analysis of ABCA1 and cholesterol efflux in HIV-infected cells / Nigora Mukhamedova [and others] -- Proteomic characterization of plasma or serum from HIV-infected patients / Nicole A. Haverland [and others] -- Proteomic characterization of exosomes from HIV-1-infected cells / Ming Li and Bharat Ramratnam -- Detecting HIV-1 Tat in cell culture supernatants by ELISA or western blot / Fabienne Rayne [and others] -- Evaluating the role of viral proteins in HIV-mediated neurotoxicity using primary human neuronal cultures / Vasudev R. Rao, Eliseo A. Eugenin, and Vinayaka R. Prasad. Measuring the uptake and transactivation function of HIV-1 Tat protein in a trans-cellular cocultivation setup / Arthur P. Ruiz and Vinayaka R. Prasad -- Evaluating the role of viral proteins in HIV-mediated neurotoxicity using primary human neuronal cultures / Vasudev R. Rao, Eliseo A. Eugenin, and Vinayaka R. Prasad.
Summary:
This third edition volume expands upon the previous editions with new research topics and numerous leading-edge HIV protocols. This book contains chapters that cover various aspects of HIV-1 replication and are helpful for investigating the mechanisms of HIV-1 replication or pathogenesis. The chapters are grouped into six sections: HIV early events; HIV-1 RNA structure and RNA-protein interactions; HIV-1 post-integration events; HIV-1 pathogenesis in animal models; tools to study HIV-1 latency; and NeuroAIDS. Chapters are written by research experts who have diligently developed techniques, improved them, simplified them, or applied them to their own HIV research. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, HIV Protocols, Third Edition is a great resource for HIV research, and contains many significant tips and tricks rarely mentioned in standard protocols.
Series:
Methods in molecular biology, 1064-3745 ; 1354 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.