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Title:

Target identification and validation in drug discovery : methods and protocols / edited by Jürgen Moll, Riccardo Colombo.

Publisher:

Humana :

Copyright Date:

c2013

Description:

xv, 371 p. : ill. (some col.) ; 26 cm.

Subject:

Drug Design--Laboratory Manuals.
Drug development.

Other Authors:

Moll, Jürgen.
Colombo, Riccardo.

Notes:

Includes bibliographical references and index.

Contents:

The path to oncology drug target validation : an industry perspective -- Identification of aptamers as specific binders and modulators of cell-surface receptor activity -- The design and structure-functional properties of DNA-based immunomodulatory sequences -- SiRNA design principles and off-target effects -- Western blot evaluation of siRNA delivery by ph-responsive peptides -- High-throughput RNAi screening for the identification of novel targets -- Integration of RNAi and small molecule screens to identify targets for drug development -- CellProfiler and KNIME : Open source tools for high content screening -- PARP inhibition as a prototype for synthetic lethal screens -- Structure-based target druggability assessment -- Validating pharmacological disruption of protein-protein interactions by acceptor photobleaching FRET imaging -- Systematic analysis of complex signal transduction pathways using protein fragment complementation assays -- Reverse phase protein microarrays and their utility in drug development -- A cell culture system that mimics chronic lymphocytic leukemia cells microenvironment for drug screening and characterization -- Two-dimensional vs. Three-dimensional in vitro tumor migration and invasion assays -- Tumor spheroid-based migration assays for evaluation of therapeutic agents -- The neurosphere assay applied to neural stem cells and cancer stem cells -- Genetically engineered animal models for in vivo target identification and validation in oncology -- Target validation in mice by constitutive and conditional RNAi -- In vivo target validation by inducible RNAi in human xenograft mouse models -- Bright-field in situ hybridization methods to discover gene amplifications and rearrangements in clinical samples -- Combined microRNA in situ hybridization and immunohistochemical detection of protein markers.

Series:

Methods in molecular biology, 1940-6029 ; v. 986
Springer protocols, 1949-2448

ISBN:

1627033106
9781627033107

OCLC:

(OCoLC)813947263

Locations:

OVUX522 -- University of Iowa Libraries (Iowa City)