Study of gap junctions in human embryonic stem cells / Alice Pebay and Raymond C.B. Wong -- Characterizing pluripotent stem sells using the TaqMan® hPSC scorecardTM panel / Jeffrey Fergus, Rene Quintanilla, and Uma Lakshmipathy -- Growth of human pluripotent stem cells using functional human extracellular matrix / Andres Sanz-Garcia, Miodrag Stojkovic, and Carmen Escobedo-Lucea -- Efficient expansion of dissociated human pluripotent stem cells using a synthetic substrate / Eihachiro Kawase -- Microarray approach to identify the signaling network responsible for self-renewal of human embryonic stem cells / Noboru Sato and Ali Brivanlou -- Monitoring stemness in long-term hESC sultures by real-time PCR / Amparo Galan and Carlos Simon -- Study of gap junctions in human embryonic stem cells / Alice Pebay and Raymond C.B. Wong -- Definitive endoderm differentiation of human embryonic stem cells combined with selective elimination of undifferentiated cells by methionine deprivation / Tomonori Tsuyama, Nobuaki Shiraki, and Shoen Kume -- Analysis of intracellular calcium signaling in human embryonic stem cells / Adrienn Pentek, Katalin Paszty, and Agota Apati -- Genetic manipulation of human embryonic stem cells / Rachel Eiges -- Genetic modification in human pluripotent stem cells by homologous recombination and CRISPR/Cas9 system / Haipeng Xue [and others] -- Use of multicolor flow cytometry for isolation of specific cell populations deriving from differentiated human embryonic stem cells / Isabella Mengarelli, Andrew Fryga, and Tiziano Barberi -- Definitive endoderm differentiation of human embryonic stem cells combined with selective elimination of undifferentiated cells by methionine deprivation / Tomonori Tsuyama, Nobuaki Shiraki, and Shoen Kume -- Directed differentiation of human embryonic stem cells into neural progenitors / Erin Banda and Laura Grabel -- Differentiation of human embryonic stem cells on periodontal ligament fibroblasts / Y. Murat Elcin, Bulend Inanc, and A. Eser Elcin -- Derivation of epithelial cells from human embryonic stem cells as an in vitro model of vocal mucosa / Vlasta Lungova, Ciara Leydon, and Susan Thibeault -- Human embryonic and hepatic stem cell differentiation visualized in two and three dimensions based on serial sections / Peter S. Vestentoft [and others] -- Derivation of chondrogenic cells from human embryonic stem cells for cartilage tissue engineering / Wei Seong Toh and Tong Cao -- Microgrooved surface modulates neuron differentiation in human embryonic stem cells / David Lu [and others] -- Directed differentiation of human embryonic stem cells into neural progenitors / Erin Banda and Laura Grabel -- Generation of megakaryocytes and platelets from human pluripotent stem cells / Marjorie Pick -- Generation of epithelial cell populations from human pluripotent stem cells using a small-molecule inhibitor of Src family kinases / Joshua A. Selekman, Xiaojun Lian, and Sean P. Palecek -- Dual-SMAD inhibition/WNT activation-based methods to induce neural crest and derivatives from human pluripotent stem cells / Stuart M. Chambers [and others] -- Accelerated three-dimensional neuroepithelium formation from human embryonic stem cells and its use for quantitative differentiation to human retinal pigment epithelium / Yu Zhu, Sven Schreiter, and Elly M. Tanaka -- Efficient production of photoreceptor precursor cells from human embryonic stem cells / Anat Yanai [and others] -- Generation of megakaryocytes and platelets from human pluripotent stem cells / Marjorie Pick -- Microgrooved surface modulates neuron differentiation in human embryonic stem cells / David Lu [and others]. Erratum to: Microgrooved surface modulates neuron differentiation in human embryonic stem cells / David Lu [and others].
Summary:
The potential of human embryonic stem cells to advance not only regenerative medicine applications but also our fundamental understanding of stem cell biology continues to drive interest in research with these cells. This detailed volume collects some of the most interesting and useful protocols that have emerged in the area over the last several years. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Human Embryonic Stem Cell Protocols, Third Edition serves as a valuable resource to all those interested in exploring stem cell biology questions in a research setting.
Series:
Methods in molecular biology, 1064-3745 ; v. 1307 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.