Isolation and culture of muscle stem cells / Chiara Mozzetta. Chromatin immunoprecipitation / Laura Wiehle and Achim Breiling -- Chromatin preparation and chromatin immuno-precipitation from Drosophila embryos / Eva Löser, Daniel Latreille, and Nicola Iovino -- ChIP-seq data processing for PcG proteins and associated histone modifications / Ozren Bogdanović and Simon J. van Heeringen -- Analysis of single-locus replication timing in asynchronous cycling cells / Lo Sardo Federica -- Noncoding RNA interplay with the genome / Davide Gabellini -- RIP : RNA immunoprecipitation / Miriam Gagliardi and Maria R. Matarazzo -- Capture hybridization analysis of DNA targets / Alec N. Sexton, Martin Machyna, and Matthew D. Simon -- Identification of RNA-protein interactions through in vitro RNA pull-down assays / Claire Barnes and Aditi Kanhere -- Understanding RNA-chromatin interactions using chromatin isolation by RNA purification (ChIRP) / Ci Chu and Howard Y. Chang -- Analysis RNA-seq and noncoding RNA / Alberto Arrigoni [and others] -- Dynamics of polycomb complexes / Daniela Palacios -- Fluorescence resonance energy transfer microscopy for measuring chromatin complex structure and dynamics / Alessandro Cherubini and Alessio Zippo -- Analysis of endogenous protein interactions of polycomb group of proteins in mouse embryonic stem cells / Lluis Morey and Luciano Di Croce -- Determination of polycomb group of protein compartmentalization through chromatin fractionation procedure / Federica Marasca, Fabrizia Marullo, and Chiara Lanzuolo -- Automatic segmentation method combining an active contour model and a classification technique for detecting polycomb-group proteins in high-throughput microscopy images / Francesco Gregoretti [and others] -- Polymer physics of the large-scale structure of chromatin / Simona Bianco [and others] -- Chromosome conformation capture in Drosophila / Hua-Bing Li -- Chromosome conformation capture in primary human cells / Alice Cortesi and Beatrice Bodega -- Determination of high-resolution 3D chromatin organization using circular chromosome conformation capture (4C-seq) / Mélody Matelot and Daan Noordermeer -- Chromosome conformation capture on chip (4C) : data processing / Benjamin Leblanc [and others] -- In vivo models to address the function of polycomb group proteins / Frédéric Bantignies -- Rapid TALEN assembly protocol / Arslan Akmammedov, Tomonori Katsuyama, and Renato Paro -- Following the motion of polycomb bodies in living Drosophila embryos / Thierry Cheutin and Giacomo Cavalli -- Reprogramming of somatic cells towards pluripotency by cell fusion / Andrzej R. Malinowski and Amanda G. Fisher -- Imaginal disc transplantation in Drosophila / Tomonori Katsuyama and Renato Paro -- Isolation and culture of muscle stem cells / Chiara Mozzetta.
Summary:
This volume presents the most recent technologies used in the Polycomb Group Proteins (PcG) field. Chapters detail state-of-the-art methods, creating a unique and comprehensive reference source for investigating Polycomb function in the nucleus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Polycomb Group Proteins: Method and Protocols aims to ensure successful results in the further study of this vital field.
Series:
Methods in molecular biology, 1064-3745 ; 1480 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.