Measurement of macrophage-specific in vivo reverse cholesterol transport in mice / Wendy Jessup, Maaike Kockx, and Leonard Kritharides. Hybrid in silico/in vitro approaches for the identification of functional cholesterol-binding domains in membrane proteins / Coralie Di Scala and Jacques Fantini -- Structural stringency of cholesterol for membrane protein function utilizing stereoisomers as novel tools : a review / Md. Jafurulla and Amitabha Chattopadhyay -- Manipulating cholesterol status within cells / Winnie Luu, Ingrid C. Gelissen, and Andrew J. Brown -- Assaying low-density-lipoprotein (LDL) uptake into cells / Anke Loregger, Jessica K. Nelson, and Noam Zelcer -- Use of L-sIDOL transgenic mice as a murine model to study hypercholestorolemia and atherosclerosis / Eser J. Zerenturk and Anna C. Calkin -- CRISPR/Cas9-mediated generation of Niemann-Pick C1 knockout cell line / Ximing Du, Ivan Lukmantara, and Hongyuan Yang -- Quantitative measurement of cholesterol in cell populations using flow cytometry and fluorescent perfringolysin O* / Jian Li, Peter L. Lee, and Suzanne R. Pfeffer -- Transport assays for sterol-binding proteins : stopped-flow fluorescence methods for investigating intracellular cholesterol transport mechanisms of NPC2 protein / Leslie A. McCauliff and Judith Storch -- Synthesis and live-cell imaging of fluorescent sterols for analysis of intracellular cholesterol transport / Maciej Modzel, Frederik W. Lund, and Daniel Wüstner -- Measurement of cholesterol transfer from lysosome to peroxisome using an in vitro reconstitution assay / Jie Luo, Ya-Cheng Liao, Jian Xiao, and Bao-Liang Song -- Measurement of mitochondrial cholesterol import using a mitochondria-targeted CYP11A1 fusion construct / Barry E. Kennedy, Mark Charman, and Barbara Karten -- Identifying sterol response elements within promoters of genes / Laura J. Sharpe and Andrew J. Brown -- Membrane extraction of HMG CoA reductase as determined by susceptibility of lumenal epitope to in vitro protease digestion / Lindsey L. Morris and Russell A. DeBose-Boyd -- Determining the topology of membrane-bound proteins using PEGylation / Vicky Howe and Andrew J. Brown -- Measuring activity of cholesterol synthesis enzymes using gas chromatography/mass spectrometry / Anika V. Prabhu, Winnie Luu, and Andrew J. Brown -- Sterol analysis by quantitative mass spectrometry / Andrew M. Jenner and Simon H.J. Brown -- Measurement of rates of cholesterol and fatty acid synthesis in vivo using tritiated water / Adam M. Lopez, Jen-Chieh Chuang, and Stephen D. Turley -- Methods for monitoring ABCA1-dependent sterol release / Yoshio Yamauchi, Shinji Yokoyama, and Ta-Yuan Chang -- ABC-transporter mediated sterol export from cells using radiolabeled sterols / Alryel Yang and Ingrid C. Gelissen -- Measurement of macrophage-specific in vivo reverse cholesterol transport in mice / Wendy Jessup, Maaike Kockx, and Leonard Kritharides.
Summary:
This volume provides state-of-the-art techniques for studying various aspects of cholesterol homeostasis, including its uptake, synthesis and efflux from the cell, as well as its trafficking within the cell. Chapters also cover techniques for studying the regulation of cholesterol homeostasis at both the transcriptional and post-translational levels, as well as studying the membrane topology and structure of cholesterol-related proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cholesterol Homeostasis: Methods and Protocol aims to provide key techniques in tackling the investigation of cholesterol homeostasis.
Series:
Methods in molecular biology, 1064-3745 ; 1583 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.