Preparation and differentiation of mesenchymal stem cells from ears of adult mice -- 3-D adipocyte differentiation and peri-adipocyte collagen turnover -- Differentiation of white and brown adipocytes from human pluripotent stem cells -- Optimal protocol for the differentiation and metabolic analysis of human adipose stromal cells -- Human adipose-derived stromal/stem cell isolation, culture, and osteogenic differentiation -- Analysis of adiopose tissue lipid using mass spectrometry -- Measurement of long-chain fatty acid uptake into adipocytes -- Measurement of the unfolded protein response to investigate its role in adipogenesis and obesity -- Application of activity-based protein profiling to study enzyme function in adipocytes -- Measurement of lipolysis -- Measurement of lipolysis products secreted by 3T3-L1 adipocytes using mcirofluidics -- Methods for performing lipidomics in white adipose tissue -- Measuring respiratory activity of adipocytes and adipose tissues in real time -- Detecting protein carbonylation in adipose tissue and in cultured adipocytes -- Use of fluorescence microscopy to probe intracellular lipolysis -- Evaluation of protein phosphorylation during adipogenesis.
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.