Generation of humanized mice for analysis of human dendritic cells / Yasuyuki Saito, Jana M. Ellegast, and Markus G. Manz. In vitro generation of human XCR1+ dendritic cells from CD34+ hematopoietic progenitors / Sreekumar Balan and Marc Dalod -- Derivation and utilization of functional CD8+ dendritic cell lines / Matteo Pigni, Devika Ashok, and Hans Acha-Orbea -- Isolation of dendritic cell progenitor and bone marrow progenitor cells from mouse / Nobuyuki Onai and Toshiaki Ohteki -- Isolation and enrichment of large numbers of highly purified mouse spleen dendritic cell populations and their in vitro equivalents / David Vremec -- Dendritic cell subset purification from human tonsils and lymph nodes / MeĢlanie Durand and Elodie Segura -- Isolation and identification of conventional dendritic cell subsets from the intestine of mice and men / Charlotte L. Scott ... [et al.] -- Isolation of human skin dendritic cell subsets / Merry Gunawan, Laura Jardine, and Muzlifah Haniffa -- Isolation of mouse dendritic cell subsets and macrophages from the skin / Camille Malosse and Sandrine Henri -- Isolation of conventional dendritic cells from mouse lungs / Lianne van de Laar, Martin Guilliams, and Simon Tavernier -- Purification of human dendritic cell subsets from peripheral blood / Solana Alculumbre and Lucia Pattarini -- Protocols for the identification and isolation of antigen-presenting cells in human and mouse tissues / Naomi McGovern ... [et al.] -- Measurement of export to the cytosol in dendritic cells using a cytofluorimetry-based assay / Omar I. Vivar, Joao G. Magalhaes, and Sebastian Amigorena -- Cross-presentation assay for human dendritic cells / Elodie Segura -- Analysis of intracellular trafficking of dendritic cell receptors for antigen targeting / Haiyin Liu ... [et al.] -- Characterization of dendritic cell subsets through gene expression analysis / Thien-Phong Vu Manh and Marc Dalod -- In vivo ablation of a dendritic cell subset expressing the chemokine receptor XCR1 / Hiroaki Hemmi, Katsuaki Hoshino, and Tsuneyasu Kaisho -- In vivo analysis of intestinal mononuclear phagocytes / Caterina Curato ... [et al.] -- In vivo imaging of cutaneous DCs in mice / Gyohei Egawa and Kenji Kabashima -- Analysis of dendritic cell function using Clec9A-DTR transgenic mice / Piotr Tetlak and Christiane Ruedl -- Analysis of DC functions using CD205-DTR knock-in mice / Tomohiro Fukaya ... [et al.] -- Generation of humanized mice for analysis of human dendritic cells / Yasuyuki Saito, Jana M. Ellegast, and Markus G. Manz.
Summary:
The third edition of this volume is aimed at providing both beginners and more experienced researchers a choice of methods to isolate and analyze dendritic cells(DC). An introductory review provides an overview of recent advances in the characterization of DC subsets in mouse and human. While additional chapters provide methods to culture human and mouse dendritic cells, protocols for the isolation of dendritic cells, the isolation of dendritic cell progenitors from mouse, and the purification of dendritic cells from human blood. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Dendritic Cell Protocols, Third Edition aims to ensure successful results in the further study of this vital field.
Series:
Methods in molecular biology, 1064-3745 ; 1423 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.