Single-cell analysis of T-cell receptor Αβ repertoire / Pradyot Dash, George C. Wang, and Paul G. Thomas -- Analyzing the effect of aging on CD8+ T-cell phenotype using flow cytometry / Min Sun Shin and Insoo Kang -- Cell-mediated immune response to influenza using ex vivo stimulation and assays of cytokine and granzyme B responses / Janet E. McElhaney and Beth Gentleman -- Assays for monitoring macroautophagy activity in T cells / Yair Botbol and Fernando Macian -- Fluorescence-based approaches for quantitative assessment of protein carbonylation, protein disulfides, and protein conformation in biological tissues / Asish R. Chaudhuri [and others] -- Monitoring the DNA damage response at dysfunctional telomeres / Rekha Rai and Sandy Chang -- Single-cell analysis of T-cell receptor Αβ repertoire / Pradyot Dash, George C. Wang, and Paul G. Thomas -- Analysis of DNA methylation by pyrosequencing / Colin Delaney, Sanjay K. Garg, and Raymond Yung. Laboratory and data analysis methods for characterization of human B cell repertoires by high-throughput DNA sequencing / Chen Wang [and others] -- Discovery of novel microRNAs in aging Caenorhabditis elegans / Alexandre de Lencastre and Frank Slack -- Analysis of DNA methylation by pyrosequencing / Colin Delaney, Sanjay K. Garg, and Raymond Yung.
Summary:
In this volume, expert researchers detail protocols for evaluating the protean effects of immunosenescence on innate and adaptive immune function, including approaches to the analysis of antigen receptor repertoire. In addition, this book guides readers through cell biology and biochemical methods for analyses of telomere dysfunction, autophagy, protein oxidation, microRNAs, and DNA methylation.
Series:
Methods in molecular biology, 1064-3745 ; 1343 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.