Fabrication of biofunctionalized, cell-laden macroporous 3D PEG hydrogels as bone marrow analogs for the cultivation of human hematopoietic stem and progenitor cells. Conjugation of proteins to polymer chains to create multivalent molecules -- Bioreactor cultivation of anatomically shaped human bone grafts -- The delivery and evaluation of RNAi therapeutics for heterotopic ossification pathologies -- Extracellular matrix mimetic peptide scaffolds for neural stem cell culture and differentiation -- Microfluidic device to culture 3D in vitro human capillary networks -- An assay to quantify chemotactic properties of degradation products from extracellular matrix -- Mimicking bone microenvironment for directing adipose tissue-derived mesenchymal stem cells into osteogenic differentiation -- Derivation and network formation of vascular cells from human pluripotent stem cells -- Cultivation of human bone-like tissue from pluripotent stem cell-derived osteogenic progenitors in perfusion bioreactors -- Biomimetic strategies incorporating enzymes into CaP coatings mimicking the in vivo environment -- Multifunction co-culture model for evaluating cell-cell interactions -- High-throughput cell aggregate culture for stem cell chondrogenesis -- Multiwell plate tools for controlling cellular alignment with grooved topography -- Fabrication of biofunctionalized, cell-laden macroporous 3D PEG hydrogels as bone marrow analogs for the cultivation of human hematopoietic stem and progenitor cells.
Summary:
Biomimetics and Stem Cells: Methods and Protocols collects a series of approaches to demonstrate the role and value of biomimetics for the better understanding of stem cell behavior and the acceleration of their application in regenerative medicine. Recent advances in tissue engineering are enabling scientists to instruct stem cells toward differentiating into the right phenotypes, in the right place and at the right time. Given these advances, biomimetic environments are being designed to recapitulate, in vitro, the combinations of factors known to guide tissue development and regeneration in vivo and thereby help unlock the full potential of the stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and essential, Biomimetics and Stem Cells: Methods and Protocols focuses on the use of biomimetic systems for stem cells in order to aid in moving this vital field of study forward.
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.