Investigation of protein-protein interactions and conformational changes in hedgehog signaling pathway by FRET / Lin Fu, Xiangdong Lv, Yue Xiong, and Yun Zhao -- In vivo imaging of hedgehog transport in Drosophila Epithelia / Irene Seijo-Barandiarán, Isabel Guerrero, and Marcus Bischoff -- Modeling hedgehog signaling through flux-saturated mechanism / Óscar Sánchez, Juan Calvo, Carmen Ibáñez, Isabel Guerrero, and Juan Soler -- Evaluating the activity of smoothened toward G proteins using [35S] guanosine 5'-(3- O -thio)triphosphate ([35S]GTPγS) / David R. Manning, Feng Shen, and Natalia A. Riobo -- Analysis of smoothened phosphorylation and activation in cultured cells and wing discs of drosophila / Kai Jiang and Jianhang Jia -- Investigation of protein-protein interactions and conformational changes in hedgehog signaling pathway by FRET / Lin Fu, Xiangdong Lv, Yue Xiong, and Yun Zhao -- Determination of acetylation of the gli transcription factors / Sonia Coni, Laura Di Magno, and Gianluca Canettieri -- Measuring expression levels of endogenous gli genes by immunoblotting and real-time PCR / Pawel Niewiadomski and Rajat Rohatgi -- Quantitative immunoblotting of endogenous hedgehog pathway components / Shohreh F. Farzan and David J. Robbins -- Measuring Gli2 phosphorylation by selected reaction monitoring mass spectrometry / Robert Ahrends, Pawel Niewiadomski, Mary N. Teruel, and Rajat Rohatgi -- Rapid screening of Gli2/3 mutants using the Flp-In system / Pawel Niewiadomski and Rajat Rohatgi -- Insights into gli factors ubiquitylation methods / Paola Infante, Romina Alfonsi, and Lucia Di Marcotullio -- Determination of acetylation of the gli transcription factors / Sonia Coni, Laura Di Magno, and Gianluca Canettieri -- Determination and analysis of cellular metabolic changes by noncanonical hedgehog signaling / Raffaele Teperino and John Andrew Pospisilik. Methods for detection of Ptc1-driven LacZ expression in adult mouse skin / Donna M. Brennan-Crispi, My G. Mahoney, and Natalia A. Riobo -- Determination and analysis of cellular metabolic changes by noncanonical hedgehog signaling / Raffaele Teperino and John Andrew Pospisilik.
Series:
Methods in molecular biology, 1064-3745 ; 1322 Springer protocols, 1949-2448
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