Quantification of five clinically important amino acids by HPLC-Triple TOF 5600 based on pre-column double derivatization method / Shuang Deng, David Scott, and Uttam Garg -- Quantification of free carnitine and acylcarnitines in plasma or serum using HPLC/MS/MS / David Scott, Bryce Heese, and Uttam Garg -- Quantification of arginine and its methylated derivatives in plasma by high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) / Faye B. Vicente [and others] -- Quantitation of albumin in urine by liquid chromatography tandem mass spectrometry / Hemamalini Ketha and Ravinder J. Singh -- Quantitation of aldosterone in serum or plasma using liquid chromatography-tandem mass spectrometry (LC- MS/MS) / J. Grace Van Der Gugten and Daniel T. Holmes -- Quantification of five clinically important amino acids by HPLC-Triple TOF 5600 based on pre-column double derivatization method / Shuang Deng, David Scott, and Uttam Garg -- Direct measurement of free estradiol in human serum and plasma by equilibrium dialysis-liquid chromatography-tandem mass spectrometry / Julie A. Ray [and others] -- Quantitation of ubiquinone (coenzyme Q10) in serum/plasma using liquid chromatography electrospray tandem mass spectrometry (ESI-LC-MS/MS) / Richard E. Mathieu Jr. and Catherine P. Riley -- Quantitative analysis of salivary cortisol using LC-MS/MS / Yan Victoria Zhang -- Quantification of dihydroxyacetone phosphate (DHAP) in human red blood cells by HPLC-TripleTOF 5600 mass spectrometer / Shuang Deng [and others] -- Simultaneous quantitation of estradiol and sstrone in serum using liquid chromatography mass spectrometry / Catherine P. Riley, Richard E. Mathieu Jr., and Carmen Wiley -- Direct measurement of free estradiol in human serum and plasma by equilibrium dialysis-liquid chromatography-tandem mass spectrometry / Julie A. Ray [and others] -- Quantification of metanephrine and normetanephrine in urine using liquid chromatography-tandem mass spectrometry / Jessica Gabler and Sihe Wang -- Quantitation of insulin analogues in serum using immunoaffinity extraction, liquid chromatography, and tandem mass spectrometry / J. Grace Van Der Gugten, Sophia Wong, and Daniel T. Holmes -- Quantitation of insulin-like growth factor 1 in serum by liquid chromatography high resolution accurate-mass mass spectrometry / Hemamalini Ketha and Ravinder J. Singh -- Quantitation of free metanephrines in plasma by liquid chromatography-tandem mass spectrometry / Courtney Heideloff, Drew Payto, and Sihe Wang -- Quantification of metanephrine and normetanephrine in urine using liquid chromatography-tandem mass spectrometry / Jessica Gabler and Sihe Wang -- Determination of phenylalanine and tyrosine by high performance liquid chromatography-tandem mass spectrometry / Judy Peat and Uttam Garg -- Quantitation of 5-methyltetrahydrofolate in cerebrospinal fluid using liquid chromatography-electrospray tandem mass spectrometry / Erland Arning and Teodoro Bottiglieri -- Quantitative organic acids in urine by two dimensional gas chromatography-time of flight mass spectrometry (GCxGC-TOFMS) / Lawrence Sweetman, Paula Ashcraft, and Jeanna Bennett-Firmin -- High sensitivity measurement of pancreatic polypeptide and its variant in serum and plasma by LC-MS/MS / Hernando Escobar [and others] -- Quantitation of parathyroid hormone in serum or plasma by liquid chromatography-tandem mass spectrometry / Hemamalini Ketha and Ravinder J. Singh -- Determination of phenylalanine and tyrosine by high performance liquid chromatography-tandem mass spectrometry / Judy Peat and Uttam Garg -- Quantification of dehydroepiandrosterone, 11-Deoxycortisol, 17-Hydroxyprogesterone, and testosterone by liquid chromatography-tandem mass spectrometry (LC/MS/MS) / Ada Munar, Clint Frazee, and Uttam Garg -- Urine pyrimidine metabolite determination by HPLC tandem mass spectrometry / Qin Sun -- Quantitation of plasma renin activity in plasma using liquid chromatography-tandem mass spectrometry (LC- MS/MS) / J. Grace Van Der Gugten and Daniel T. Holmes -- Quantitation of S-adenosylmethionine and S-adenosylhomocysteine in plasma using liquid chromatography electrospray tandem mass spectrometry / Erland Arning and Teodoro Bottiglieri -- Simple, high-throughput method for analysis of ceramide, glucosylceramide, and ceramide trihexoside in dried blood spots by LC/MS/MS / Wei-Lien Chuang, Joshua Pacheco, and Kate Zhang -- Quantification of dehydroepiandrosterone, 11-Deoxycortisol, 17-Hydroxyprogesterone, and testosterone by liquid chromatography-tandem mass spectrometry (LC/MS/MS) / Ada Munar, Clint Frazee, and Uttam Garg -- Quantitation of 25-OH-Vitamin-D2 and 25-OH-Vitamin-D3 in urine using LC-MS/MS / Dean C. Carlow, Ryan C. Schofield, and Michelle Denburg. Quantification of 1,25-Dihydroxyvitamin D2 and D3 in serum using liquid chromatography-tandem mass spectrometry / Jonathon Mahlow, Dustin R. Bunch, and Sihe Wang -- High-throughput serum 25-hydroxy vitamin D testing with automated sample preparation / Judy Stone -- Quantitation of 25-OH-Vitamin-D2 and 25-OH-Vitamin-D3 in urine using LC-MS/MS / Dean C. Carlow, Ryan C. Schofield, and Michelle Denburg.
Summary:
This volume provides stepwise instructions for the analysis of numerous clinically important analytes by mass spectrometry. Mass spectrometry offers clinical laboratory scientists a number of advantages including increased sensitivity and specificity, multiple component analysis, and no need for specialized reagents. The techniques described are a must for the measurement of many clinically relevant analytes in the fields of drug analysis, endocrinology, and inborn errors of metabolism. Each chapter provides a brief introduction about a specified analyte, followed by detailed instructions on the analytical protocol. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and practical, Clinical Applications of Mass Spectrometry in Biomolecular Analysis: Methods and Protocols is a great resource for clinical laboratory scientists who are already using or thinking of bringing mass spectrometry to their laboratories.
Series:
Methods in molecular biology, 1064-3745 ; 1378 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.