"Published In: United States, 13 October 2016"--Distributor's website. Includes bibliographical references and index.
Contents:
Efficient induction and isolation of human primordial germ cell-like cells from competent human pluripotent stem cells / Naoko Irie and M. Azim Surani. Methods for studying the germline of the human parasite Schistosoma mansoni / Julie N.R. Collins and James J. Collins III -- Evaluation of the asymmetric division of Drosophila male germline stem cells / Mayu Inaba and Yukiko M. Yamashita -- Live imaging of the Drosophila testis stem cell niche / Leah J. Greenspan and Erika L. Matunis -- RNA isolation from early Drosophila larval ovaries / Dana Gancz and Lilach Gilboa -- Live-cell imaging of the adult Drosophila ovary using confocal microscopy / Nevine A. Shalaby and Michael Buszczak -- Measurement of mRNA Poly(A) tail lengths in Drosophila female germ cells and germ-line stem cells / Aymeric Chartier, Willy Joly, and Martine Simonelig -- Identification of germ-line stem cells in zebrafish / Bruce W. Draper -- Primordial germ cell isolation from Xenopus laevis embryos / Amanda M. Butler ... [et al.] -- Single-cell lineage analysis of oogenesis in mice / Lei Lei and Allan C. Spradling -- Visualization and lineage tracing of Pax7+ spermatogonial stem cells in the mouse / Gina M. Aloisio ... [et al.] -- Transplantation as a quantitative assay to study mammalian male germline stem cells / Aileen R. Helsel and Jon M. Oatley -- Mouse fetal germ cell isolation and culture techniques / Cassy M. Spiller, Guillaume Burnet, and Josephine Bowles -- Rattus norvegicus spermatogenesis colony-forming assays / F. Kent Hamra -- Identification of mouse piRNA pathway components using anti-MIWI2 antibodies / Takamasa Hirano, Hidetoshi Hasuwa, and Haruhiko Siomi -- Efficient induction and isolation of human primordial germ cell-like cells from competent human pluripotent stem cells / Naoko Irie and M. Azim Surani.
Summary:
This second edition provides updated and new chapters on selected genetic, molecular, biochemical, and cell biological techniques. Chapter's guide readers through methods and principles on primordial germ cells and germline stem cells, however many of these principles can be applied to different types of adult stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Germline Stem Cells: Second Edition aims to present the new findings and techniques that have developed since the first edition.
Series:
Methods in molecular biology, 1064-3745 ; 1463 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.