Generation of targeted mutations in zebrafish using the CRISPR/cas system. Detection and quantification of VEGF isoforms by ELISA -- Quantitation of circulating neuropilin-1 in human, monkey, mouse, and rat sera by ELISA -- Detection and quantification of vascular endothelial growth factor receptor tyrosine kinases in primary human endothelial cells -- Induction of VEGF secretion in cardiomyocytes by mechanical stretch -- Chromatin immunoprecipitation assay: Examining the interaction of NFkB with the VEGF promoter -- An overview of vegf-mediated signal transduction -- Identification of receptor tyrosine kinase inhibitors using cell surface biotinylation and affinity isolation -- Analysis of VEGF-mediated ERK5 activity in endothelial cells -- In vitro angiogenesis assays -- Chemotactic migration of endothelial cells towards VEGF-A165 -- Vasculogenesis and angiogenesis in VEGF receptor-1 deficient mice -- The embryonic mouse hindbrain and postnatal retina as in vivo models to study angiogenesis -- VEGF gene transfer to the utero-placental circulation of pregnant guinea pigs to enhance fetal growth -- VEGF gene transfer to the utero-placental circulation of pregnant sheep to enhance fetal growth -- Generation of targeted mutations in zebrafish using the CRISPR/cas system.
Series:
Methods in molecular biology, 1064-3745 ; 1332 Springer protocols, 1949-2448
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