Part 1. Sample preparation strategies for proteomics. Proteomes, their compositions and their sources -- Protein fractionation and enrichment prior to proteomics sample preparation -- Sample preparation for mass spectrometry-based proteomics; from proteomes to peptides -- Plant structure and specificity -- challenges and sample preparation considerations for proteomics -- Improving proteome coverage by reducing sample complexity via chromatography -- part 2. Mass spectrometry for proteomics analysis. Database search engines: paradigms, challenges and solutions -- Mass analyzers and mass spectrometers -- Top-down mass spectrometry: proteomics to proteoforms -- part 3. Bioinformatic tools for proteomicsdata analysis and interpretation. Platforms and pipelines for proteomics data analysis and management -- Tandem mass spectrum sequencing: an alternative to database search engines in shotgun proteomics -- Visualization, inspection and interpretation of shotgun proteomics identification results -- Protein inference -- Modification site localization in peptides -- Useful web resources -- Mass spectrometry-based protein quantification -- Bioinformatics tools for proteomics data interpretation -- part 4. Applications of proteomics technologies in biological and medical sciences. Identification, quantification, and site localization of protein posttranslational modifications via mass spectrometry-based proteomics -- Protein-protein interaction detection via mass spectrometry-based proteomics -- Protein structural analysis via mass spectrometry-based proteomics -- part 5. Clinical proteomics. Introduction to clinical proteomics -- Discovery of candidate biomarkers -- Statistical approaches to candidate biomarker panel selection -- Qualification and verification of protein biomarker candidates -- Protocol for standardizing high-to-moderate abundance protein biomarker assessments through an MRM-with-standard-peptides quantitative approach -- Index.
Series:
Advances in experimental medicine and biology, 0065-2598 ; volume 919
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.