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Title:

DNA damage detection in situ, ex vivo, and in vivo : methods and protocols / edited by Vladimir V. Didenko.

Publisher:

Humana Press,

Copyright Date:

c2011

Description:

xiii, 313 p. : ill. (some col.) ; 27 cm.

Subject:

DNA damage.
DNA damage--Methodology.--Methodology.
DNA--Analysis.
DNA Damage--Laboratory Manuals.
DNA--analysis--Laboratory Manuals.
Microbiological Techniques--Laboratory Manuals.

Other Authors:

Didenko, Vladimir V. edt

Notes:

Includes bibliographical references and index.

Contents:

In situ detection of apoptosis by the TUNEL assay : an overview of techniques / Deryk T. Loo -- Combination of TUNEL assay with immunohistochemistry for simultaneous detection of DNA fragmentation and oxidative cell damage / Alexander E. Kalyuzhny -- EM-ISEL : a useful tool to visualize DNA damage at the ultrastructural level / Antonio Migheli -- In situ labeling of DNA breaks and apoptosis by T7 DNA polymerase / Vladimir V. Didenko -- In situ ligation : a decade and a half of experience / Peter J. Hornsby and Vladimir V. Didenko -- In situ ligation simplified : using PCR fragments for detection of double-strand DNA breaks in tissue sections / Vladimir V. Didenko -- 5'OH DNA breaks in apoptosis and their labeling by topoisomerase-based approach / Vladimir V. Didenko -- Detection of DNA strand breaks in apoptotic cells by flow- and image-cytometry / Zbigniew Darzynkiewicz and Hong Zhao --
Fluorochrome-labeled inhibitors of caspases : convenient in vitro and in vivo markers of apoptotic cells for cytometric analysis / Zbigniew Darzynkiewicz ... [et al.] -- Combining fluorescent in situ hybridization with the comet assay for targeted examination of DNA damage and repair / Sergey Shaposhnikov, Preben D. Thomsen, and Andrew R. Collins -- Simultaneous labeling of single- and double-strand DNA breaks by DNA breakage detection-FISH (DBD-FISH) / José Luis Fernández, Dioleyda Cajigal, and Jaime Gosálvez -- Co-localization of DNA repair proteins with UV-induced DNA damage in locally irradiated cells / Jennifer Guerrero-Santoro, Arthur S. Levine, and Vesna Rapić-Otrin -- Ultrasound imaging of apoptosis : spectroscopic detection of DNA-damage effects at high and low frequencies / Roxana M. Vlad, Michael C. Kolios, and Gregory J. Czarnota --
Quantifying etheno-DNA adducts in human tissues, white blood cells, and urine by ultrasensitive ³²P-postlabeling and immunohistochemistry / Jagadeesan Nair ... [et al.] -- ELISpot assay as a tool to study oxidative stress in peripheral blood mononuclear cells / Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny -- Cytokinesis-block micronucleus cytome assay in lymphocytes / Philip Thomas and Michael Fenech -- Buccal micronucleus cytome assay / Philip Thomas and Michael Fenech -- [Gamma]-H2AX detection in peripheral blood lymphocytes, splenocytes, bone marrow, xenografts, and skin / Christophe E. Redon ... [et al.] -- Immunologic detection of benzo(a)pyrene-DNA adducts / Regina M. Santella and Yu-Jing Zhang -- Non-invasive assessment of oxidatively damaged DNA : liquid chromatography-tandem mass spectrometry analysis of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine / Vilas Mistry ... [et al.] --
Assessing sperm DNA fragmentation with the sperm chromatin dispersion test / José Luis Fernández ... [et al.]

Series:

Methods in molecular biology, 1064-3745 ; v. 682
Springer protocols

ISBN:

1603274081 (hbk. : alk. paper)
9781603274081 (hbk. : alk. paper)

OCLC:

(OCoLC)648099788

LCCN:

2010938360

Locations:

OVUX522 -- University of Iowa Libraries (Iowa City)