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Title:
Human retroviruses : methods and protocols / edited by Elisa Vicenzi and Guido Poli.
Publisher:
Humana Press,
Copyright Date:
2014
Description:
xvii, 345 pages : illustrations (some colored) ; 26 cm.
Subject:
Retroviridae--Laboratory Manuals.
HIV--immunology--Laboratory Manuals.
Retroviruses--Laboratory manuals.
HIV (Viruses)--Laboratory manuals.
HTLV (Viruses)--Laboratory manuals.
Laboratory Manuals.
Other Authors:
Vicenzi, Elisa, editor.
Poli, Guido, editor.
Springer Science+Business Media, copyright holder.
Notes:
Includes bibliographical references and index.
Contents:
Virion attachment and entry : HIV gp120 env biotinylation, gp120 env, or integrin ligand-binding assay / Claudia Cicala and James Arthos -- CryoEM analysis of capsid assembly and structural changes upon interactions with a host restriction factor, TRIM5[alpha] / Gongpu Zhao and Peijun Zhang -- The fate of HIV-1 capsid : a biochemical assay for HIV-1 uncoating / Yang Yang, Jeremy Luban, and Felipe Diaz-Griffero -- The cyclosporin A washout assay to detect HIV-1 uncoating in infected cells / Amy E. Hulme and Thomas J. Hope -- Imaging HIV-1 nuclear pre-integration complexes / Anna Cereseto and Mauro Giacca -- HIV-1 reverse transcription / Andrea Cimarelli and Jean-Luc Darlix -- RNase H : specificity mechanisms of action, and antiviral target / Karin Moelling, Felix Broecker, and John E. Kerrigan -- HIV-1 chromatin, transcription, and the regulatory protein Tat / Laurence Colin, Eric Verdin, and Carine Van Lint --
HIV-1 Rev function and RNA nuclear-cytoplasmic export / Alan Cochrane -- HIV-1 accessory proteins : Nef / Anke Heigele [and three others] -- HIV-1 accessory proteins : VpR / Richard Y. Zho and Michael I. Bukrinsky -- HIV-1 accessory proteins : Vpu and Vif / Amy Andrew and Klaus Strebel -- SIVSM/HIV-2 Vpx proteins : function and uses in the infection of primary myeloid cells / Gregory Berger and Andrea Cimarelli -- Imaging of HIV assembly and release / Barbara Müller and Jacomine Krijnse-Locker -- HIV-1 isolation from infected peripheral blood mononuclear cells / Stefania Dispinseri [and four others] -- Determination of HIV-1 co-receptor usage / Mariangela Cavarelli and Gabriella Scarlatti -- The macrophage and HIV : basic concepts and methodologies / Suzanne Gartner -- HIV infection of dendritic cells / Najla Nasr [and three others] -- Histocultures (tissue explants) in human retrovirology / Anush Arakelyan [and four others] --
Single-copy quantification of HIV-1 in clinical samples / Ann Wiegand and Frank Maldarelli -- Quantification of total HIV1-DNA in peripheral blood mononuclear cells / Christine Rouzioux, Adeline Mélard, and Véronique Avéttand-Fénoël -- HIV-1 based lentiviral vectors / Ying Poi Liu and Ben Berkhout -- Quantification of miRNA by poly(A)-RT-qPCR arrays and verification of target sites in HIV-1 using a one-LTR infectious molecular clone / Zachary A. Klase, Laurent Houzet, and Kuan-Teh Jeang -- Investigating human T cell lymphotropic retrovirus (HTLV) Tax function with molecular and immunophenotypic techniques / Greta Forlani, Roberto S. Accolla, and Giovanna Tosi -- Proviral load determination of HTLV-1 and HTLV-2 in patients' peripheral blood mononuclear cells by real-time PCR / Claudio Casoli, Elisabetta Pilotti, and Umberto Bertazzoni --
Quantitative analysis of human T-lymphotropic virus type 1 (HTLV-1) gene expression using nucleo-cytoplasmic fractionation and splice junction-specific real-time RT-PCR (qRT-PCR) / Ilaria Cavallari, Francesca Rende, and Vincenzo Ciminale.
Series:
Methods in molecular biology, 1064-3745 ; 1087
Springer protocols, 1949-2448
ISBN:
9781627036696 (alk. paper)
1627036695 (alk. paper)
OCLC:
(OCoLC)855905421
LCCN:
2013949423
Locations:
OVUX522 -- University of Iowa Libraries (Iowa City)

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