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Title:

T cell protocols / edited by Gennaro De Libero.

Edition:

2nd ed.

Publisher:

Springer distributor,

Copyright Date:

2009

Description:

xi, 165 p. : ill. ; 27 cm.

Subject:

T cells.
T-Lymphocytes--Laboratory Manuals.

Other Authors:

De Libero, Gennaro.

Notes:

Includes bibliographical references and index.

Contents:

Analysis of frequency and phenotype of antigen-specific T cells / Angus Stock and Vincenzo Cerundolo -- B cell helper assays / Sergio Abrignani, ... [et al.] -- Transkingdom RNA interference (tkRNAi) / Thu A. Nguyen and Johannes H. Fruehauf -- Flow cytometry and cell activation / Sonia Gavasso -- Investigating T cells by polychromatic flow cytometry / Enrico Lugli, Leonarda Troiano, and Andrea Cossarizza -- Generation of human T cell clones / Sabrina Mariotti and Roberto Nisini -- Limiting dilution analysis of antigen-specific T cells / Jorge Carneiro, Lurdes Duarte, and Elisabetta Padovan -- T cell epitope-mapping by cytokine gene expression assay / Maurizio Provengano and Giulio C. Spagnoli -- Cytokine multiplex immunoassay / Wilco de Jager, Berent Prakken, and Ger T. Rijkers -- Purification of the T cell antigen receptor and analysis by blue-native PAGE / Mahima Swamy and Wolfgang W.A. Schamel -- Non-replicating recombinant vaccinia virus expressing CD80 to enhance T-cell stimulation / Paul Zajac.

Series:

Methods in molecular biology ; 514
Springer protocols

ISBN:

1588295877 (hbk. : alk. paper)
9781588295873 (hbk. : alk. paper)

Locations:

USUX851 -- Iowa State University - Parks Library (Ames)

OVUX522 -- University of Iowa Libraries (Iowa City)