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Title:

Enzyme engineering : methods and protocols / edited by James C. Samuelson.

Publisher:

Humana Press ;

Copyright Date:

c2013

Description:

xi, 252 p. : ill. (some col.) ; 27 cm.

Subject:

Enzymes--Biotechnology.
Enzymes--Industrial applications.
Protein engineering.
Enzymes--Laboratory Manuals.
Protein Engineering--methods--Laboratory Manuals.
Biomedical Engineering--methods--Laboratory Manuals.

Other Authors:

Samuelson, James C., 1968- edt

Notes:

Includes bibliographical references and index.

Contents:

Tripartite fusion system for the selection of protein variants with increased stability in vivo / Linda Foit and James C.A. Bardwell -- Determining enzyme kinetics via isothermal titration calorimetry / Neil A. Demarse ... [et al.] -- GFP reporter screens for the engineering of amino acid degrading enzymes from libraries expressed in bacteria / Olga Paley ... [et al.] -- Flow cytometric assays for interrogating LAGLIDADG homing endonuclease DNA-binding and cleavage properties / Sarah K. Baxter ... [et al.] -- TAL effector nuclease (TALEN) engineering / Ting Li and Bing Yang -- In vitro evolution of enzymes / Misha V. Golynskiy ... [et al.] -- Residue-specific incorporation of unnatural amino acids into proteins in vitro and in vivo / Amrita Singh-Blom, Randall A. Hughes, and Andrew D. Ellington -- Reconstructing evolutionary adaptive paths for protein engineering / Megan F. Cole ... [et al.] --
Oligonucleotide recombination enabled site-specific mutagenesis in bacteria / Bryan M. Swingle -- FX cloning : a versatile high-throughput cloning system for characterization of enzyme variants / Eric R. Geertsma -- Use of Sulfolobus solfataricus PCNA subunit proteins to direct the assembly of multimeric enzyme complexes / Hidehiko Hirakawa and Teruyuki Nagamune -- Gene synthesis by assembly of deoxyuridine-containing oligonucleotides / Romualdas Vaisvila and Jurate Bitinaite -- Protein engineering : single or multiple site-directed mutagenesis / Pei-Chung Hsieh and Romualdas Vaisvila -- Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination / Nili Ostrov, Laura M. Wingler, and Virginia W. Cornish -- Promiscuity-based enzyme selection for rational directed evolution experiments / Sandeep Chakraborty ... [et al.] -- Rational protein sequence diversification by multi-codon scanning mutagenesis / Jia Liu and T. Ashton Cropp --
Screening libraries for improved solubility : using E. coli dihydrofolate reductase as a reporter / Jian-Wei Liu and David L. Ollis -- In vitro directed evolution of enzymes expressed by E. coli in microtiter plates / Bradley J. Stevenson, Sylvia H.-C. Yip, and David L. Ollis.

Series:

Methods in molecular biology, 1064-3745 ; 978
Springer protocols, 1949-2448

ISBN:

1627032924 (alk. paper)
9781627032926 (alk. paper)

OCLC:

(OCoLC)813947662

LCCN:

2013930234

Locations:

OVUX522 -- University of Iowa Libraries (Iowa City)