PTEN: history of a tumor suppressor / Leonardo Salmena -- PTEN at 18 : still growing / Olena Gorbenko and Vuk Stambolic -- Immunopathologic assessment of PTEN expression / Mireia Castillo-Martin, Tin Htwe Thin, Ana Collazo Lorduy, and Carlos Cordon-Cardo -- Measurement of PTEN by flow cytometry / Jolm F. Woolley and Leonardo Salmena -- Detecting PTEN and PI3K signaling in brain / Guo Zhu and Suzanne J. Baker -- Germline PTEN mutation analysis for PTEN hamartoma tumor syndrome / Joanne Ngeow and Charis Eng -- Methods for assessing the in vivo role of PTEN in glucose homeostasis / Cynthia T. Luk, Stephanie A. Schroer, and Minna Woo -- Rapid detection of dynamic PTEN regulation in living cells using intramolecular BRET / Stanislas Misticone, Evelyne Lima-Fernandes, and Mark G.H. Scott -- Methods for the identification of PTEN-targeting MicroRNAs / Andrea Tuccoli, Marianna Vitiello, Andrea Marranci, Francesco Russo, and Laura Poliseno -- Posttranscriptional regulation of PTEN by competing endogenous RNAs / Yvonne Tay and Pier Paolo Pandolfi -- In cell and in vitro assays to measure PTEN ubiquitination / Amit Gupta, Helene Maccario, Nisha Kriplani, and Nicholas R. Leslie -- Assessing PTEN subcellular localization / Anabel Gil, José I. López, and Rafael Pulido -- Methods to study PTEN in mitochondria and endoplasmic reticulum / Sonia Missiroli, Claudia Morganti, Carlotta Giorgi, and Paolo Pinton -- Methods in the study of PTEN structure : X-ray crystallography and hydrogen deuterium exchange mass spectrometry / Glenn R. Masson, John E. Burke, and Roger L. Williams -- Methods for PTEN in stem cells and cancer stem cells / Suzanne Schubbert, Jing Jiao, Marcus Ruscetti, Jonathan Nakashima, Shumin Wu, Hong Lei, Qinzhi Xu, Wenkai Yi, Haichuan Zhu, and Hong Wu -- Modeling cancer-associated mutations of PTEN in mice / Antonella Papa and Pier Paolo Pandolfi -- C. elegans methods to study PTEN / Shanqing Zheng and Ian D. Chin-Sang.
Summary:
This volume provides detailed descriptions of prevailing and novel techniques used by experts in the study of PTEN function in disease and biology.
Series:
Methods in molecular biology, 1064-3745 ; 1388 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.