505-00 GWA-portal : genome-wide association studies made easy / €Umit Seren. Phenotyping crop root crowns : general guidance and specific protocols for maize, wheat, and soybean / Larry M. York -- Developmental analysis of Arabidopsis root meristem / Riccardo Di Mambro and Sabrina Sabatini -- Genetic and phenotypic analysis of lateral root development in Arabidopsis thaliana / Selene Napsucialy-Mendivil and Joseph G. Dubrovsky -- Adapting the lateral root-inducible system to Medicago truncatula / Violaine Herrbach, Fabienne Maillet, and Sandra Bensmihen -- Characterization of root epidermal cell patterning and differentiation in Arabidopsis / Jorge E. Salazar-Henao, Girish Mokkapati, Eunice Han Xian Khor, Yi-Chia Chou, Wann-Neng Jane, and Wolfgang Schmidt -- In vitro assay for induction of adventitious rooting on intact Arabidopsis hypocotyls / Hoang Khai Trinh, Inge Verstraeten, and Danny Geelen -- Root gravitropism : quantification, challenges, and solutions / Lukas Muller, Malcolm J. Bennett, Andy French, Darren M. Wells, and Ranjan Swarup -- Calcium ion dynamics in roots : imaging and analysis / Ellie Himschoot, Melanie Krebs, Alex Costa, Tom Beeckman, and Steffen Vanneste-- Optimized whole-mount in situ immunolocalization for Arabidopsis thaliana root meristems and lateral root primordia / Michael Karampelias, Ricardo Tejos, Jirı Friml, and Steffen Vanneste -- Light sheet fluorescence microscopy optimized for long-term imaging of Arabidopsis root development / Paolo Baesso, Ricardo S. Randall, and Giovanni Sena -- Histological profiling over time to optimize root cell type-specific reporter lines for cell sorting / Beatriz Lagunas, Liam Walker, Rana Muhammad Fraz Hussain, Ian Hands-Portman, Kate Woolley-Allen, and Miriam L. Gifford -- Long-term in vivo imaging of luciferase-based reporter gene expression in Arabidopsis roots / Wei Xuan, Davy Opdenacker, Steffen Vanneste, and Tom Beeckman -- Cortical cell length analysis during gravitropic root growth / Maria Scholler, Jurgen Kleine-Vehn, and Elena Feraru -- Growth rate normalization method to assess gravitropic root growth / Maria Scholler, Elizabeth Sarkel, Jurgen Kleine-Vehn, and Elena Feraru -- Immunoprecipitation of membrane proteins from Arabidopsis thaliana root tissue / Sascha Waidmann, Lucinda De-Araujo, Jurgen Kleine-Vehn, and Barbara Korbei -- Monitoring transcriptomic changes in soil-grown roots and shoots of Arabidopsis thaliana subjected to a progressive drought stress / Khurram Bashir, Sultana Rasheed, Akihiro Matsui, Kei Iida, Maho Tanaka, and Motoaki Seki -- Chromatin immunoprecipitation sequencing (ChIP-Seq) for transcription factors and chromatin factors in Arabidopsis thaliana roots : from material collection to data analysis / Sandra Cortijo, Varodom Charoensawan, Francois Roudier, and Philip A. Wigge -- μChIP-Seq for genome-wide mapping of in vivo TF-DNA interactions in Arabidopsis root protoplasts / Alessia Para, Ying Li, and Gloria M. Coruzzi -- Proteome analysis of Arabidopsis roots / Natalia Nikonorova, Lam Dai Vu, Elisabeth Stes, Kris Gevaert, and Ive De Smet -- Step-by-step construction of gene Co-expression networks from high-throughput Arabidopsis RNA sequencing data / Orlando Contreras-Lopez, Tomas C. Moyano, Daniela C. Soto, and Rodrigo A. Gutierrez -- GWA-portal : genome-wide association studies made easy / €Umit Seren.
Summary:
This detailed volume provides diverse elegant methods, complemented with existing protocols, which are optimized for the current needs in plant root biology as well as for use in plant species other than Arabidopsis thaliana. The collection covers methods ranging from genetic screens, phenotypic analysis, and cell biology methods to systems biology tools and genome-wide approaches. The collection contains a range of complexity from fundamental methods for quantification of different root developmental processes to complex methods that require sophisticated equipment. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Root Development: Methods and Protocols serves as a valuable tool for plant biologists specialized in root development, from beginners to experienced researchers in the field.
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.