Probing conformational changes in rhodopsin using hydrogen-deuterium exchange coupled to mass spectrometry / Tivadar Orban and Yaroslav Tsybovsky -- Rhodopsin purification from dark-adapted bovine retina / Elise Blankenship and David T. Lodowski -- Mammalian expression, purification, and crystallization of rhodopsin variants / Daniel Mattle [and 4 others] -- Imaging of rhodopsin crystals with two-photon microscopy / Grazyna Palczewska and David Salom -- Functional stability of rhodopsin in a bicelle system : evaluating G protein activation by rhodopsin in bicelles / Ali I. Kaya, T. M. Iverson, and Heidi E. Hamm -- Rhodopsin-arrestin-1 interaction in bicelles / Qiuyan Chen [and 7 others] -- Detection of structural waters and their role in structural dynamics of rhodopsin activation / Liwen Wang and Mark R. Chance -- Probing conformational changes in rhodopsin using hydrogen-deuterium exchange coupled to mass spectrometry / Tivadar Orban and Yaroslav Tsybovsky -- Quantification of arrestin-rhodopsin binding stoichiometry / Beata Jastrzebska -- Investigation of rhodopsin dynamics in its signaling state by solid-state deuterium NMR spectroscopy / Andrey V. Struts [and 3 others] -- Sequential structural changes in rhodopsin occurring upon photoactivation / Naoki Kimata [and 4 others.] -- Dynamic single-molecule force spectroscopy of rhodopsin in native membranes / Paul S. -H. Park and Daniel J. Mùˆller -- High-resolution atomic force microscopy imaging of Rhodopsin in rod outer segment disk membranes / Patrick D. Bosshart, Andreas Engel, and Dimitrios Fotiadis -- Detection of rhodopsin dimerization in situ by PIE-FCCS, a time-resolved fluorescence spectroscopy / Adam W. Smith -- Oligomeric state of rhodopsin within rhodopsin-transducin complex probed with succinylated concanavalin A / Beata Jastrzebska -- Quantification of arrestin-rhodopsin binding stoichiometry / Peter H. Tang and Rosalie K. Crouch -- Sustained delivery of retinoids to prevent photoreceptor death / Daniele Dell'Orco -- Three-dimensional architecture of murine rod cilium revealed by cryo-EM / Theodore G. Wenseland Jared C. Gilliam -- Monitoring of rhodopsin trafficking and mistrafficking in live photoreceptors / Kerrie H. Lodowski and Yoshikazu Imanishi -- Measurements of rhodopsin diffusion within signaling membrane microcompartments in live photoreceptors / Mehdi Najafi and Peter D. Calvert -- Kinetics of rhodopsin's chromophore monitored in a single photoreceptor / Leopold Adler IV [and 3 others] -- Supplementation with vitamin A derivatives to rescue vision in animal models of degenerative retinal diseases / Lindsay Perusek, Akiko Maeda, and Tadao Maeda -- Sustained delivery of retinoids to prevent photoreceptor death / Peter H. Tang and Rosalie K. Crouch -- Gene therapy to rescue retinal degeneration caused by mutations in rhodopsin / Brian P. Rossmiller, Renee C. Ryals, and Alfred S. Lewin. Gene therapy to rescue retinal degeneration caused by mutations in rhodopsin / Brian P. Rossmiller, Renee C. Ryals, and Alfred S. Lewin.
Summary:
This volume explores the technological improvements in protein engineering, expression, purification, and crystallization of several rhodopsin photoactive intermediates, thus increasing our understanding of rhodopsin activation. The first chapters of the book focus on methods developed to study fundamentals of rhodopsin structure and function, starting with improved purification protocols of native and mutated rhodopsin, followed by methods used for rhodopsin reconstitution into lipid bilayers stabilizing rhodopsin function properties, and finally describing recently developed methods to study structural dynamics of rhodopsin activation and its mechanistic properties. Subsequently, chapters underline various techniques that have been developed to visualize the rhodopsin dimer and to study its functional significance. The next few chapters highlight cutting-edge imaging techniques of photoreceptors, rhodopsin trafficking, and its diffusion within signaling membranes. Finally the book concludes with recent developments that could be potentially beneficial in patient treatments, and treatment strategies for retinal degenerative diseases.Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Timely and practical, Rhodopsin: Methods and Protocols reaches out to researchers and health practitioners, and provides timely protocol useful for studying structural and functional properties of rhodopsin.
Series:
Methods in molecular biology, 1064-3745 ; 1271 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.