Methods for the characterization of plant-growth promoting rhizobacteria / Md. Motaher Hossain and Farjana Sultana. Bacterial genotyping methods : from the basics to modern / Margarita M. Ochoa-Dıaz, Silvana Daza-Giovannetty, and Doris G omez-Camargo -- Real-time reverse transcription PCR as a tool to study virulence gene regulation in bacterial pathogens / Gili Aviv and Ohad Gal-Mor -- Usage of a bioluminescence reporter system to image promoter activity during host infection / Gili Aviv and Ohad Gal-Mor -- lacZ reporter system as a tool to study virulence gene regulation in bacterial pathogens Gili Aviv and Ohad Gal-Mor -- Western blotting against tagged virulence determinants to study bacterial pathogenicity / Gili Aviv and Ohad Gal-Mor -- Molecular methods to analyze the effect of proteins expressed by Salmonella during its intracellular stage / Carlos Medina, Beatriz Mesa-Pereira, Eva M. Camacho, Amando Flores, and Eduardo Santero -- Organoids as a model to study infectious disease / Kristen A. Engevik, Andrea L. Matthis, Marshall H. Montrose, and Eitaro Aihara -- Surface proteome biotinylation combined with bioinformatic tools as a strategy for predicting pathogen interacting proteins / Anita Horvatic, Josipa Kules, Nicolas Guillemin, Franjo Martinkovic, Iva Stimac, Vladimir Mrljak, and Mangesh Bhide -- Systems biology modeling to study pathogen-host interactions / Muberra Fatma Cesur and Saliha Durmus -- Phage therapy : various perspectives on how to improve the art / Stephen T. Abedon -- Application of RNA-seq and bioimaging methods to study microbe-microbe interactions and their effects on biofilm formation and gene expression / Cristina Isabel Amador, Claus Sternberg, and Lars Jelsbak -- Serial dilution-based growth curves and growth curve synchronization for high-resolution time series of bacterial biofilm growth / Fernando Govantes -- Detection of bacterial quorum sensing molecules / Elke Stein and Adam Schikora -- Generating chromosome-located transcriptional fusions to fluorescent proteins for single-cell gene expression Analysis in Pseudomonas syringae / Jose S. Rufian, Diego Lopez-Marquez, Nieves Lopez-Pagan, Murray Grant, Javier Ruiz-Albert, and Carmen R. Beuzon -- Introduction of genetic material in Ralstonia solanacearum through natural transformation and conjugation / Anthony Perrier, Patrick Barberis, and Stephane Genin -- In vitro and in vivo secretion/translocation assays to identify novel Ralstonia solanacearum type 3 effectors / Fabien Lonjon, Nemo Peeters, Stephane Genin, and Fabienne Vailleau -- Plant pathogenicity phenotyping of Ralstonia solanacearum strains / Arry Morel, Nemo Peeters, Fabienne Vailleau, Patrick Barberis, Gaofei Jiang, Richard Berthome, and Alice Guidot -- Methods to quantify biotic-induced stress in plants / Marcel Bach-Pages and Gail M. Preston -- From sample to data : preparing, obtaining, and analyzing images of plant-pathogen interactions using confocal microscopy / Helen N. Fones and George R. Littlejohn -- Screening of c-di-GMP-regulated exopolysaccharides in host interacting bacteria / Jochen Schmid, Broder Ruhmann, Volker Sieber, Lorena Romero-Jimenez, Juan Sanjuan, and Daniel Perez-Mendoza -- Primary characterization of small RNAs in symbiotic nitrogen-fixing gacteria / Marta Robledo, Natalia I. Garcia-Tomsig, and Jose I. Jimenez-Zurdo -- New, nondestructive, split-root system for local and systemic plant responses studies with soybean / Angeles Hidalgo, Jose E. Ruiz-Sainz, and Jose M. Vinardell -- Methods for the characterization of plant-growth promoting rhizobacteria / Md. Motaher Hossain and Farjana Sultana.
Summary:
This detailed volume explores numerous multidisciplinary approaches employed to analyze the role of different molecules or strategies used by different guests to survive inside hosts. Divided into several sections, the book delves into animal-pathogen interaction, microbe-microbe interaction, as well as plant-pathogen interaction. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.uthoritative and practical, Host-Pathogen Interactions: Methods and Protocols provides many molecular and bioinformatics techniques currently in use at reputed laboratories in countries all around the world, vital for many kinds of host-pathogen interactions studies.
Series:
Methods in molecular biology, 1064-3745 ; 1734 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.