Previous edition: 2009. Includes bibliographical references and index.
Contents:
Detection of interaction between toll-like receptors and other transmembrane proteins by co-immunoprecipitation assay / Yu-Ran Lee, Wondae Kang, and You-Me Kim -- Bioinformatic analysis of toll-like receptor sequences and structures / Tom P. Monie, Nicholas J. Gay, and Monique Gangloff -- Toll-like receptor interactions measured by microscopic and flow cytometric FRET / Gabor L. Horvath, Pia Langhoff, and Eicke Latz -- Using confocal microscopy to investigate intracellular trafficking of toll-like receptors / Harald Husebye and Sarah L. Doyle -- Assessing the inhibitory activity of oligonucleotides on TLR7 sensing / Jonathan Ferrand and Michael P. Gantier -- Methods for delivering DNA to intracellular receptors / Katryn J. Stacey [and others] -- Detection of interaction between toll-like receptors and other transmembrane proteins by co-immunoprecipitation assay / Yu-Ran Lee, Wondae Kang, and You-Me Kim -- TLR function in murine CD4+ T lymphocytes and their role in inflammation / Stephanie Flaherty and Joseph M. Reynolds -- Measuring monomer-to-filament transition of MAVS as an in vitro activity assay for RIG-I-Like receptors / Bin Wu, Yu-San Huoh, and Sun Hur -- Co-transcriptomic analysis by RNA sequencing to simultaneously measure regulated gene expression in host and bacterial pathogen / Timothy Ravasi [and others] -- Simple methods to investigate microRNA induction in response to toll-like receptors / Victoria G. Lyons and Claire E. McCoy -- Determining the function of long noncoding RNA in innate immunity / Susan Carpenter -- Analysis of post-transcriptional gene regulation of nod-like receptors via the 3'UTR / Moritz Haneklaus -- TLR function in murine CD4+ T lymphocytes and their role in inflammation / Stephanie Flaherty and Joseph M. Reynolds -- Allergens and activation of the toll-like receptor response / Tom P. Monie and Clare E. Bryant -- Toll-like receptor-dependent immune complex activation of B cells and dendritic cells / Krishna L. Moody [and others] -- Analysis of TLR-induced metabolic changes in dendritic cells using the seahorse XFe96 extracellular flux analyzer / Leonard R. Pelgrom, Alwin J. van der Ham, and Bart Everts -- Toll-like receptor signalling and the control of intestinal barrier function / Daniel G.W. Johnston and SineĢad C. Corr -- Understanding the role of cellular molecular clocks in controlling the innate immune response / Anne M. Curtis and Caio T. Fagundes -- Methods to investigate the role of toll-like receptors in allergic contact dermatitis / Marc Schmidt, Matthias Goebeler, and Stefan F. Martin -- Allergens and activation of the toll-like receptor response / Tom P. Monie and Clare E. Bryant -- Investigating the role of toll-like receptors in mouse models of gastric cancer / Alison C. West and Brendan J. Jenkins. Delineating the role of toll-like receptors in the neuro- inflammation model EAE / Francesca Fallarino [and others] -- Use of miRNA antagonists in the alleviation of inflammatory disorders / Lucien P. Garo and Gopal Murugaiyan -- Investigating the role of toll-like receptors in mouse models of gastric cancer / Alison C. West and Brendan J. Jenkins.
Summary:
Annotation This second edition volume expands on the first edition with new developments on Toll-Like Receptors (TLRs) controlling events such as cross-priming of associated pattern recognition receptors, post-transcriptional regulation, interaction with other cellular and biologic systems, and cancer progression. This book is divided into five sections: Part I outlines methods for TLR detection, interaction, and intracellular trafficking; Part II describes methods and assays to investigate how TLRs cross-prime other pattern recognition receptors, including intracellular DNA receptors and inflammasome formation; Part III highlights RNA regulation, detailing how TLRs can induce RNA transcripts and molecules such as lncRNAs and microRNAs; Part IV explores TLR detection and activation in systems such as epithelial barrier function, metabolism and the circadian clock, as well as cellular systems including T and B lymphocytes; and Part V describes models to delineate the role of TLRs in diseases such as dermatitis, arthritis, and gastric cancer. Written in the highly successful "Methods in Molecular Biology "series format, each chapter contains a summary, a list of required materials, step-by-step, readily reproducible laboratory protocols, useful notes to investigate TLRs in cell culture, systems and disease, and tips on troubleshooting and avoiding known pitfalls.Practical and cutting-edge, "Toll-Like Receptors: Methods and Protocols, Second Edition" is a valuable resource to any immunologist, molecular or medical biologist working in a laboratory setting. It will add skill to both students and the more advanced molecular biologist who wishes to learn a new technique or move to a different area within their current repertoire of practical knowledge.
Series:
Methods in molecular biology, 1064-3745 ; 1390 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.