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Title:
Noncanonical amino acids : methods and protocols / edited by Edward A. Lemke.
Publisher:
Humana Press :
Copyright Date:
2018
Description:
xi, 411 pages : illustrations (some color) ; 26 cm.
Subject:
Amino acids.
Amino Acids.
Laboratory Manuals.
Other Authors:
Lemke, Edward A., editor.
Notes:
Includes bibliographical references and index.
Contents:
Genetic encoding of unnatural amino acids in C. elegans / Lloyd Davis and Sebastian Greiss. Artificial division of codon boxes for expansion of the amino acid repertoire of ribosomal polypeptide synthesis / Yoshihiko Iwane, Takayuki Katoh, Yuki Goto, and Hiroaki Suga -- Cell-free protein synthesis for multiple site-specific incorporation of noncanonical amino acids using cell extracts from RF-1 deletion E. coli strains / Eiko Seki, Tatsuo Yanagisawa, and Shigeyuki Yokoyama -- Tub-tag labeling ; chemoenzymatic incorporation of unnatural amino acids / Jonas Helma, Heinrich Leonhardt, Christian P.R. Hackenberger, and Dominik Schumacher -- Directed evolution of orthogonal pyrrolysyl-tRNA synthetases in Escherichia coli for the genetic encoding of noncanonical amino acids / Moritz J. Schmidt and Daniel Summerer -- Genetic code expansion in enteric bacterial pathogens / Huangtao Zheng, Shixian Lin, and Peng R. Chen -- Self-directed in cell production of methionine analogue azidohomoalanine by synthetic metabolism and its incorporation into model proteins / Ying Ma, Martino L. Di Salvo, and Nediljko Budisa -- Residue-specific incorporation of noncanonical amino acids for protein engineering / Mark B. van Eldijk and Jan C. M. van Hest -- Using amber and ochre nonsense codons to code two different noncanonical amino acids in one protein gene / Jeffery M. Tharp and Wenshe R. Liu -- Genetic incorporation of unnatural amino acids into proteins of interest in Streptomyces venezuelae ATCC 15439 / Jingxuan He and Charles E. Melançon III -- Expression and purification of site-specifically lysine-acetylated and natively-folded proteins for biophysical investigations / Michael Lammers -- Site-specific incorporation of sulfotyrosine using an expanded genetic code / Xiang Li and Chang C. Liu -- Site-specific protein labeling with tetrazine amino acids / Robert J. Blizzard, True E. Gibson, and Ryan A. Mehl -- Mapping of protein interfaces in live cells using genetically encoded crosslinkers / Lisa Seidel and Irene Coin -- Generation of stable amber suppression cell lines / Simon J. Elsässer -- Trapping chromatin interacting proteins with genetically encoded, UV-activatable crosslinkers in vivo / Christian Hoffmann, Heinz Neumann, and Petra Neumann-Staubitz -- Genetically encoding unnatural amino acids in neurons in vitro and in the embryonic mouse brain for optical control of neuronal proteins / Ji-Yong Kang, Daichi Kawaguchi, and Lei Wang -- Genetic code expansion- and click chemistry-based site-specific protein labeling for intracellular DNA-PAINT imaging / Ivana Nikić-Spiegel -- MultiBacTAG-genetic code expansion using the baculovirus expression system in Sf21 cells / Christine Koehler and Edward A. Lemke -- Production and chemoselective modification of adeno-associated virus site-specifically incorporating an unnatural amino acid residue into its capsid / Rachel E. Kelemen, Sarah B. Erickson, and Abhishek Chatterjee -- Generation of intramolecular FRET probes via noncanonical amino acid mutagenesis / Simone Brand and Yao-Wen Wu -- Fluorogenic tetrazine-siliconrhodamine probe for the labeling of noncanonical amino acid tagged proteins / Eszter Kozma, Giulia Paci, Gemma Estrada Girona, Edward A. Lemke, and Péter Kele -- Site-specific protein labeling utilizing lipoic acid ligase (LplA) and bioorthogonal inverse electron demand diels-alder reaction / Mathis Baalmann, Marcel Best, and Richard Wombacher -- Genetic encoding of unnatural amino acids in C. elegans / Lloyd Davis and Sebastian Greiss.
Series:
Methods in molecular biology, 1064-3745 ; v. 1728
Springer protocols, 1949-2448
ISBN:
9781493975730
1493975730
OCLC:
(OCoLC)1005187961
LCCN:
2017964669
Locations:
OVUX522 -- University of Iowa Libraries (Iowa City)

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