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03113aam a2200325 a 4500 001 0F6D4A62E2DD11E284C630D7DAD10320 003 SILO 005 20130702010114 008 121021s2013 nyua b 001 0 eng d 020 $a 1627033106 020 $a 9781627033107 035 $a (OCoLC)813947263 040 $a BTCTA $b eng $c BTCTA $d UKMGB $d YDXCP $d EYM $d GZM $d COO $d NUI $d SILO 050 4 $a QH506 $b .M45 v.986 245 00 $a Target identification and validation in drug discovery : $b methods and protocols / $c edited by JuÌrgen Moll, Riccardo Colombo. 260 $a New York : $b Humana : $c c2013. 300 $a xv, 371 p. : $b ill. (some col.) ; $c 26 cm. 490 1 $a Methods in molecular biology, $x 1940-6029 ; $v v. 986 490 1 $a Springer protocols, $x 1949-2448 504 $a Includes bibliographical references and index. 505 0 $a The path to oncology drug target validation : an industry perspective -- Identification of aptamers as specific binders and modulators of cell-surface receptor activity -- The design and structure-functional properties of DNA-based immunomodulatory sequences -- SiRNA design principles and off-target effects -- Western blot evaluation of siRNA delivery by ph-responsive peptides -- High-throughput RNAi screening for the identification of novel targets -- Integration of RNAi and small molecule screens to identify targets for drug development -- CellProfiler and KNIME : Open source tools for high content screening -- PARP inhibition as a prototype for synthetic lethal screens -- Structure-based target druggability assessment -- Validating pharmacological disruption of protein-protein interactions by acceptor photobleaching FRET imaging -- Systematic analysis of complex signal transduction pathways using protein fragment complementation assays -- Reverse phase protein microarrays and their utility in drug development -- A cell culture system that mimics chronic lymphocytic leukemia cells microenvironment for drug screening and characterization -- Two-dimensional vs. Three-dimensional in vitro tumor migration and invasion assays -- Tumor spheroid-based migration assays for evaluation of therapeutic agents -- The neurosphere assay applied to neural stem cells and cancer stem cells -- Genetically engineered animal models for in vivo target identification and validation in oncology -- Target validation in mice by constitutive and conditional RNAi -- In vivo target validation by inducible RNAi in human xenograft mouse models -- Bright-field in situ hybridization methods to discover gene amplifications and rearrangements in clinical samples -- Combined microRNA in situ hybridization and immunohistochemical detection of protein markers. 650 2 $a Drug Design $v Laboratory Manuals. $2 [MeSH] 650 0 $a Drug development. 700 1 $a Moll, JuÌrgen. 700 1 $a Colombo, Riccardo. 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 986. $x 1064-3745 830 0 $a Springer protocols (Series) 941 $a 1 952 $l OVUX522 $d 20180110023248.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=0F6D4A62E2DD11E284C630D7DAD10320Initiate Another SILO Locator Search