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04073aam a2200409 a 4500 001 3DE3A9D6065511E8AD8CF06897128E48 003 SILO 005 20180131010242 008 090613t20092009nyua b 001 0 eng d 010 $a 2009933592 020 $a 1607612313 020 $a 9781607612315 035 $a (OCoLC)401153915 040 $a BTCTA $c BTCTA $d YDXCP $d CUS $d BWX $d TEF $d COU $d UtOrBLW $d SILO 050 4 $a QH506 $b .M45 v.577 245 00 $a Reverse chemical genetics : $b methods and protocols / $c edited by Hisashi Koga. 264 1 $a New York, NY : $b Humana Press, $c [2009] 300 $a xi, 272 pages : $b illustrations (some color) ; $c 27 cm. 490 1 $a Springer protocols 490 1 $a Methods in molecular biology, $x 1064-3745 ; $v 577 504 $a Includes bibliographical references and index. 505 0 $a ORFeome cloning / Osamu Ohara -- Systematic cloning of an ORFeome using the gateway system / Akihisa Matsuyama and Minoru Yoshida -- High-throughput construction of ORF clones for production of the recombinant proteins / Hisashi Yamakawa -- Computational overview of GPCR gene universe to support reverse chemical genomics study / Makiko Suwa and Yukiteru Ono -- High-performance gene expression module analysis tool and its application to chemical toxicity data / Wataru Fujibuchi ... [et al.] -- Human protein reference database and human proteinpedia as discovery tools for systems biology / T.S. Keshava Prasad, Kumaran Kandasamy, and Akhilesh Pandey -- High-throughput production of the recombinant proteins expressed in Escherichia coli utilizing cDNA resources / Kiyo Shimada and Hisashi Koga -- An insect cell-free system for recombinant protein expresion using cDNA resources / Takashi Syuzuki ... [et al.] -- 505 8 $a Recombinant protein production by a kaiko-baculovirus system / Hidekazu Nagaya -- Pulse-chase experiment for the analysis of protein stability in cultured mammalian cells by covalent fluorescent labeling of fusion proteins / Kei Yamaguchi ... [et al.] -- Application of 2D-DIGE in cancer proteomics toward personalized medicine / Tadashi Kondo and Setsuo Hirohashi -- Fully automated two-dimensional electrophoresis system for high-throughput protein analysis / Atsunori Hiratsuka and Kenji Yokoyama -- Large-scale whole mount in situ hybridization of mouse embryos / Hirohito Shimizu, Kenta Uchibe, and Hiroshi Asahara -- Identification of the specific binding proteins of bioactive small coumpound using affinity resins / Akito Tanaka -- Protein microarrays : effective tools for the study of inflammatory diseases / Xiaobo Yu ... [et al.] -- Preparation of highly sensitive protein array using reactive polymer / Toshifumi Shiroya ... [et al.] -- 505 8 $a High-throughput SPR biosensor / Motoki Kyo ... [et al.] -- Drug discovery through functional screening in the Drosophila heart / Takeshi Akasaka and Karen Ocorr -- Permeable cell assay : a method for high-throughput measurement of cellular ATP synthetic activity / Kiyotaka Y. Hara -- Novel methodology for immobilization of biomolecules on the surface of a photoresponsible polymer containing azobenzene moiety / Osamu Watanabe. 530 $a Also available online. 650 0 $a Biochemical genetics. $0 http://id.loc.gov/authorities/subjects/sh85014121 650 12 $a Combinatorial Chemistry Techniques $x methods. $2 [MeSH] $0 https://id.nlm.nih.gov/mesh/D020650Q000379 650 12 $a Genetic Techniques. $2 [MeSH] $0 https://id.nlm.nih.gov/mesh/D005821 650 22 $a Molecular Biology $x methods. $2 [MeSH] $0 https://id.nlm.nih.gov/mesh/D008967Q000379 655 2 $a Laboratory Manuals. 700 1 $a Koga, Hisashi. $0 http://id.loc.gov/authorities/names/n83142607 773 18 $w 990001282000202771 $g no:577 830 0 $a Springer protocols (Series) $0 http://id.loc.gov/authorities/names/no2008180772 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 577. $0 http://id.loc.gov/authorities/names/n92002874 941 $a 1 952 $l OVUX522 $d 20180131033224.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=3DE3A9D6065511E8AD8CF06897128E48Initiate Another SILO Locator Search