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03831aam a2200445 i 4500 001 9516810E580511E8A8F83C5097128E48 003 SILO 005 20180515010114 008 170726t20182018nyua b 001 0deng d 010 $a 2017954499 020 $a 1493974009 020 $a 9781493974009 035 $a (OCoLC)1012305664 040 $a YDX $b eng $e rda $c YDX $d CHVBK $d OCLCO $d YDX $d OCLCO $d OCLCA $d OCLCF $d PUL $d OCLCO $d GZM $d OCLCO $d STF $d NUI $d SILO 245 00 $a Cancer stem cells : $b methods and protocols / $c edited by Gianpaolo Papaccio, Vincenzo Desiderio. 264 1 $a New York : $b Humana Press, $c [2018] 300 $a xii, 223 pages : $b illustrations (some color) ; $c 27 cm. 490 1 $a Methods in molecular biology, $x 1064-3745 ; $v 1692 490 1 $a Springer protocols, $x 1949-2448 504 $a Includes bibliographical references and index. 520 $a This detailed volume gathers a comprehensive collection of methods, protocols, and procedures used for the identification, characterization, and selection of cancer stem cells. These include surface marker expression, side population, spheres formation, ALDH activity, and numerous others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert tips and troubleshooting advice to avoid potential pitfalls.iÌmprehensive and practical, Cancer Stem Cells: Methods and Protocols provides researchers with the techniques they will need in order to pursue vital research into this complex field of study. 650 0 $a Cancer cells $v Laboratory manuals. 650 0 $a Stem cells $v Laboratory manuals. 650 7 $a Cancer cells. $2 fast $0 (OCoLC)fst00845563 650 7 $a Stem cells. $2 fast $0 (OCoLC)fst01132975 650 2 $a Neoplastic Stem Cells. 650 2 $a Stem Cells. 650 2 $a Neoplasms $x therapy. 655 7 $a Laboratory manuals. $2 lcgft 655 7 $a Laboratory manuals. $2 fast $0 (OCoLC)fst01920776 700 1 $a Papaccio, Gianpaolo, $e editor. 700 1 $a Desiderio, Vincenzo, $e editor. 776 08 $i Electronic version: $t Cancer stem cells. $d New York, NY : Humana Press, 2018 $z 9781493974016 $w (OCoLC)1005923479 773 18 $w 990001282000202771 $g v:1692 880 0 $6 505-00 $a Introduction to Cancer Stem Cells: Past, Present, and Future -- Surface Markers for the Identification of Cancer Stem Cells -- The Role of CD44 and Cancer Stem Cells -- Evaluation and Isolation of Cancer Stem Cells Using ALDH Activity Assay -- Isolation of Cancer Stem Cells by Side Population Method -- Self-Renewal and CSCs In Vitro Enrichment: Growth as Floating Spheres -- In Vitro Tumorigenic Assay: The Tumor Spheres Assay -- In Vitro Tumorigenic Assay: Colony Forming Assay for Cancer Stem Cells -- Xenograft as In Vivo Experimental Model -- How to Assess Drug Resistance in Cancer Stem Cells -- Tumor Tissue Analogs for Assessment of Radioresistance in Cancer Stem Cells -- Generation of In Vitro Model of Epithelial Mesenchymal Transition (EMT) Via the Expression of a Cytoplasmic Mutant Form of Promylocytic Leukemia Protein (PML) -- Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System -- Determination of miRNAs沯m Cancer Stem Cells Using a Low Density Array Platform -- Assessing DNA Methylation in Cancer Stem Cells -- Histones Acetylation and Cancer Stem Cells (CSCs) -- Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods -- Circulating Tumor Cells. 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v 1692, $x 1064-3745 830 0 $a Springer protocols (Series), $x 1949-2448 941 $a 1 952 $l OVUX522 $d 20181116051720.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=9516810E580511E8A8F83C5097128E48Initiate Another SILO Locator Search