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03980aam a2200397 i 4500 001 0ACE02C2ECCF11E5A64DF5B3DAD10320 003 SILO 005 20160318010059 008 151204t20152015nyua b 001 0deng 010 $a 2015946748 020 $a 1493929623 (alk. paper) 020 $a 9781493929627 (alk. paper) 035 $a (OCoLC)939397119 040 $a DNLM $b eng $e rda $c NLM $d NUI $d SILO 042 $a pcc 066 $c (S 245 00 $a Immunosenecence : $b methods and protocols / $c edited by Albert C. Shaw. 264 1 $a New York : $b Humana Press/Springer, $c [2015] 300 $a xi, 270 pages : $b illustrations (some color) ; $c 27 cm. 490 1 $a Methods in molecular biology, $x 1064-3745 ; $v 1343 490 1 $a Springer protocols, $x 1949-2448 504 $a Includes bibliographical references and index. 520 $a In this volume, expert researchers detail protocols for evaluating the protean effects of immunosenescence on innate and adaptive immune function, including approaches to the analysis of antigen receptor repertoire. In addition, this book guides readers through cell biology and biochemical methods for analyses of telomere dysfunction, autophagy, protein oxidation, microRNAs, and DNA methylation. 505 80 $t Single-cell analysis of T-cell receptor Îβ repertoire / $r Pradyot Dash, George C. Wang, and Paul G. Thomas -- $t Analyzing the effect of aging on CD8+ T-cell phenotype using flow cytometry / $r Min Sun Shin and Insoo Kang -- $t Cell-mediated immune response to influenza using ex vivo stimulation and assays of cytokine and granzyme B responses / $r Janet E. McElhaney and Beth Gentleman -- $t Assays for monitoring macroautophagy activity in T cells / $r Yair Botbol and Fernando Macian -- $t Fluorescence-based approaches for quantitative assessment of protein carbonylation, protein disulfides, and protein conformation in biological tissues / $r Asish R. Chaudhuri [and others] -- $t Monitoring the DNA damage response at dysfunctional telomeres / $r Rekha Rai and Sandy Chang -- $t Single-cell analysis of T-cell receptor Îβ repertoire / $r Pradyot Dash, George C. Wang, and Paul G. Thomas -- 505 80 $t Analysis of DNA methylation by pyrosequencing / $r Colin Delaney, Sanjay K. Garg, and Raymond Yung. $t Laboratory and data analysis methods for characterization of human B cell repertoires by high-throughput DNA sequencing / $r Chen Wang [and others] -- $t Discovery of novel microRNAs in aging Caenorhabditis elegans / $r Alexandre de Lencastre and Frank Slack -- $t Analysis of DNA methylation by pyrosequencing / $r Colin Delaney, Sanjay K. Garg, and Raymond Yung. 650 0 $a Immunology. 650 0 $a Medicine. 650 12 $a Immunosenescence $x physiology $2 [MeSH] 650 22 $a Clinical Laboratory Techniques $x methods $2 [MeSH] 700 1 $a Shaw, Albert C., $e editor. 880 00 $6 505-00 $t Imaging immunosenescence / $r Feng Qian and Ruth R. Montgomery -- $t Flow cytometry analysis of NK cell phenotype and function in aging / $r Raquel Tarazona [and others] -- $t Flow cytometric identification of fibrocytes in the human circulation / $r Xinyuan Hu, Erin M. DeBiasi, and Erica L. Herzog -- $t Experimental approaches to tissue injury and repair in advanced age / $r Aleah L. Brubaker, Stewart R. Carter, and Elizabeth J. Kovacs -- $t Multicolor digital flow cytometry in human translational immunology / $r Samit R. Joshi, Subhasis Mohanty, and Albert C. Shaw -- $t Flow cytometry-based methods to characterize immune senescence in nonhuman primates / $r Christine Meyer [and others] -- $t Multiparameter phenotyping of human PBMCs using mass cytometry / $r Michael D. Leipold, Evan W. Newell, and Holden T. Maecker -- $t Imaging immunosenescence / $r Feng Qian and Ruth R. Montgomery -- 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 1343. $x 1064-3745 830 0 $a Springer protocols (Series) $x 1949-2448 941 $a 1 952 $l OVUX522 $d 20180131021602.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=0ACE02C2ECCF11E5A64DF5B3DAD10320Initiate Another SILO Locator Search