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04262aam a2200373 i 4500 001 5EA42DD6F50B11E7A6193F6E97128E48 003 SILO 005 20180109010327 008 160402t20162017nyua b 001 0deng d 010 $a 2016945156 020 $a 1493940155 020 $a 9781493940158 035 $a (OCoLC)945949254 040 $a BTCTA $b eng $e rda $c BTCTA $d YDXCP $d IDL $d OCLCO $d OCLCA $d NUI $d UtOrBLW $d SILO 050 4 $a QH506 $b .M45 v.1463 245 00 $a Germline stem cells / $c edited by Michael Buszczak. 250 $a Second edition. 264 1 $a New York : $b Humana Press, $c [2016] 300 $a xi, 229 pages : $b illustrations (some color) ; $c 26 cm. 490 1 $a Methods in molecular biology, $x 1064-3745 ; $v 1463 490 1 $a Springer protocols, $x 1949-2448 500 $a "Published In: United States, 13 October 2016"--Distributor's website. 504 $a Includes bibliographical references and index. 505 00 $t Efficient induction and isolation of human primordial germ cell-like cells from competent human pluripotent stem cells / $r Naoko Irie and M. Azim Surani. $t Methods for studying the germline of the human parasite Schistosoma mansoni / $r Julie N.R. Collins and James J. Collins III -- $t Evaluation of the asymmetric division of Drosophila male germline stem cells / $r Mayu Inaba and Yukiko M. Yamashita -- $t Live imaging of the Drosophila testis stem cell niche / $r Leah J. Greenspan and Erika L. Matunis -- $t RNA isolation from early Drosophila larval ovaries / $r Dana Gancz and Lilach Gilboa -- $t Live-cell imaging of the adult Drosophila ovary using confocal microscopy / $r Nevine A. Shalaby and Michael Buszczak -- $t Measurement of mRNA Poly(A) tail lengths in Drosophila female germ cells and germ-line stem cells / $r Aymeric Chartier, Willy Joly, and Martine Simonelig -- $t Identification of germ-line stem cells in zebrafish / $r Bruce W. Draper -- $t Primordial germ cell isolation from Xenopus laevis embryos / $r Amanda M. Butler ... [et al.] -- $t Single-cell lineage analysis of oogenesis in mice / $r Lei Lei and Allan C. Spradling -- $t Visualization and lineage tracing of Pax7+ spermatogonial stem cells in the mouse / $r Gina M. Aloisio ... [et al.] -- $t Transplantation as a quantitative assay to study mammalian male germline stem cells / $r Aileen R. Helsel and Jon M. Oatley -- $t Mouse fetal germ cell isolation and culture techniques / $r Cassy M. Spiller, Guillaume Burnet, and Josephine Bowles -- $t Rattus norvegicus spermatogenesis colony-forming assays / $r F. Kent Hamra -- $t Identification of mouse piRNA pathway components using anti-MIWI2 antibodies / $r Takamasa Hirano, Hidetoshi Hasuwa, and Haruhiko Siomi -- $t Efficient induction and isolation of human primordial germ cell-like cells from competent human pluripotent stem cells / $r Naoko Irie and M. Azim Surani. 520 $a This second edition provides updated and new chapters on selected genetic, molecular, biochemical, and cell biological techniques. Chapter's guide readers through methods and principles on primordial germ cells and germline stem cells, however many of these principles can be applied to different types of adult stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Germline Stem Cells: Second Edition aims to present the new findings and techniques that have developed since the first edition. 650 0 $a Stem cells $v Laboratory manuals. 700 1 $a Buszczak, Michael, $e editor. $0 http://id.loc.gov/authorities/names/nb2016022418 710 2 $a Springer Science+Business Media, $e copyright holder. $0 http://id.loc.gov/authorities/names/n2005003639 773 18 $w 990001282000202771 $g v:1463 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 1463. $x 1064-3745 $0 http://id.loc.gov/authorities/names/n92002874 830 0 $a Springer protocols (Series). $x 1949-2448 $0 http://id.loc.gov/authorities/names/no2008180772 941 $a 1 952 $l OVUX522 $d 20180710045057.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=5EA42DD6F50B11E7A6193F6E97128E48Initiate Another SILO Locator Search