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02120cam a22004094a 4500 001 9E912A362B0A11DE8AF32708A8D7520A 003 SILO 005 20100201083435 008 050927s2005 njua b 001 0 eng 010 $a 2005281585 020 $a 9780471453291 020 $a 0471453293 035 $a (OCoLC)58830118 040 $a DLC $c DLC $d SILO $d BAKER $d ABC $d VAM $d VPI $d NLM $d AGL $d IXA $d YDXCP $d OCLCQ $d BTCTA $d LVB $d UMM $d IWO $d SILO 042 $a pcc 082 00 $a 571.6/381 $2 22 082 04 $a 571.5381 $2 22 100 1 $a Freshney, R. Ian. 245 1 $a Culture of animal cells : $b a manual of basic technique / $c R. Ian Freshney. 250 $a 5th ed. 260 $a Hoboken, N.J. : $b Wiley-Liss, $c c2005. 300 $a 642 p. : $b ill. (some col.) ; $c 29 cm. 504 $a Includes bibliographical references (p. 581-626) and index. 505 0 $a Introduction -- Training programs -- Biology of cultured cells -- Laboratory design and layout -- Equipment -- Aseptic technique -- Safety, bioethics, and validation -- Culture vessels and substrates -- Defined media and supplements -- Serum-free media -- Preparation and sterilization -- Primary culture -- Subculture and cell lines -- Cloning and selection -- Cell separation -- Characterization -- Differentiation -- Transformation and immortalization -- Contamination -- Cryopreservation -- Quantitation -- Cytotoxicity -- Culture of specific cell types -- Culture of tumor cells -- Organotypic culture -- Scale-up -- Specialized techniques -- Problem solving -- In conclusion. 650 0 $a Tissue culture $v Laboratory manuals. 650 0 $a Cell culture $v Laboratory manuals. 650 12 $a Cells, Cultured. 650 22 $a Culture Techniques. 650 6 $a Tissus (Histologie) $x Culture $v Manuels de laboratoire. 650 6 $a Cellules $x Culture $v Manuels de laboratoire. 941 $a 4 952 $l USUX851 $d 20220602022318.0 952 $l OVUX522 $d 20180124013824.0 952 $l UWAX953 $d 20090701080000.0 952 $l PTAX572 $d 20090701080000.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=9E912A362B0A11DE8AF32708A8D7520A 994 $a 02 $b IWOInitiate Another SILO Locator Search