Monitoring the ATM-mediated DNA damage response in the cerebellum using organotypic cultures / Efrat Tal and Yosef Shiloh. Assaying for radioresistant DNA synthesis, the hallmark feature of the intra-S-phase checkpoint using a DNA fiber technique / Amanda W. Kijas and Martin F. Lavin -- ATM gene mutation detection techniques and functional analysis / Guillaume Rieunier, Catherine Dubois D'Enghien, Alice Fievet, Dorine Bellanger, Dominique Stoppa-Lyonnet, and Marc-Henri Stern -- HTRF assay for the protein kinase ATM / Phillip Adams, Jonathan Clark, Simon Hawdon, Jennifer Hill, and Andrew Plater -- ATM kinase inhibitors : HTS cellular imaging assay using cellomics ArrayScan VTI platform / Catherine Bardelle and Joanna Boros -- Image-based high content screening : automating the quantification process for DNA damage-induced foci / Yi Chieh Lim -- Analyzing ATM function by electroporation of endonucleases and immunofluorescence microscopy / Keiji Suzuki -- Quantitative and dynamic imaging of ATM kinase activity by bioluminescence imaging / Shyam Nyati, Grant Young, Brian Dale Ross, and Alnawaz Rehemtulla -- Zn(II)-Phos-Tag SDS-PAGE for separation and detection of a DNA damage-related signaling large phosphoprotein / Eiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike -- Identification of ATM protein kinase phosphorylation sites by mass spectrometry / Mark E. Graham, Martin F. Lavin, and Sergei V. Kozlov -- Studies of ATM kinase activity using engineered ATM sensitive to ATP analogues (ATM-AS) / Masato Enari, Yuko Matsushima-Hibiya, Makoto Miyazaki, and Ryo Otomo -- Functional characterization of ATM kinase using acetylation-specific antibodies / Yingli Sun and Fengxia Du -- Identification of ATM-interacting proteins by co-immunoprecipitation and Glutathione-S-Transferase (GST) pull-down assays / Amanda L. Bain, Janelle L. Harris, and Kum Kum Khanna -- ATM activation and H2AX phosphorylation induced by genotoxic agents assessed by flow- and laser scanning cytometry / Hong Zhao, H. Dorota Halicka, Jorge Garcia, Jiangwei Li, and Zbigniew Darzynkiewicz -- Peptide immunoaffinity enrichment with targeted mass spectrometry : application to quantification of ATM kinase phospho-signaling / Jeffrey R. Whiteaker, Lei Zhao, Regine M. Schoenherr, Jacob J. Kennedy, Richard G. Ivey, and Amanda G. Paulovich -- Mass spectrometry-based proteomics for quantifying DNA damage-induced phosphorylation / Marina E. Borisova, Sebastian A. Wagner, and Petra Beli -- Statistical analysis of ATM-dependent signaling in quantitative mass spectrometry phosphoproteomics / Ashley J. Waardenberg -- ChIP technique to study protein dynamics at defined DNA double strand breaks / Jie Wen and Patrick Concannon -- Studies of the DNA damage response by using the Lac Operator/Repressor (LacO/LacR) tethering system / Rossana Piccinno, Marta Cipinska, and Vassilis Roukos -- Imaging of fluorescently tagged ATM kinase at the sites of DNA double strand breaks / Anthony J. Davis, Shih-Ya Wang, David J. Chen, and Benjamin P.C. Chen -- Live cell imaging to study real-time ATM-mediated recruitment of DNA repair complexes to sites of ionizing radiation-induced DNA damage / Burkhard Jakob and Gisela Taucher-Scholz -- Analyzing heterochromatic DNA double strand break (DSB) repair in response to ionizing radiation / Karolin Klement and Aaron A. Goodarzi -- Phenotypic analysis of ATM protein kinase in DNA double-strand break formation and repair / Elisabeth Mian and Lisa Wiesmüller -- Monitoring DNA repair consequences of ATM signaling using simultaneous fluorescent readouts / Adrian Wiegmans -- Noncanonical ATM activation and signaling in response to transcription-blocking DNA damage / Jurgen A. Marteijn, Wim Vermeulen, and Maria Tresini -- Study of ATM phosphorylation by Cdk5 in neuronal cells / Hua She and Zixu Mao -- DNA damage response in human stem cells and neural descendants / Jason M. Beckta, Bret R. Adams, and Kristoffer Valerie -- Patient-specific stem cell model to investigate the neurological phenotype observed in ataxia-telangiectasia / Romal Stewart, Gautam Wali, Chris Perry, Martin F. Lavin, Francois Féron, Alan Mackay-Sim, and Ratneswary Sutharsan -- Lentiviral reprogramming of A-T patient fibroblasts to induced pluripotent stem cells / Sam Nayler, Sergei V. Kozlov, Martin F. Lavin, and Ernst Wolvetang -- Monitoring the ATM-mediated DNA damage response in the cerebellum using organotypic cultures / Efrat Tal and Yosef Shiloh.
Summary:
Ataxia-telangiectasia (A-T) is a rare and severe genetic disorder affecting children. A-T is a multisystem disease characterized by progressive neurodegeneration, immunodeficiency and cancer predisposition. This detailed volume explores the ever expanding field of research into the ATM (ataxia-telangiectasia, mutated) gene and the role played by ATM kinase in DNA damage signaling and diverse cellular processes. What follows is a handy desktop reference for both seasoned A-T researchers and postgraduate students, as it demonstrates the breadth of recent developments in A-T studies. Written for the highly successfulMethods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Both classic and cutting-edge techniques are described, including ATM gene mutation detection, assays for radiosensitivity and radioresistant DNA synthesis, new methods to measure ATM kinase activity by imaging microscopy and high content screening as well as proteomics, phosphoproteomics and bioinformatics approaches to decipher ATM-dependent signalling pathways. Additional methods include generation of patient-specific stem cells and approaches to study ATM functions in the nervous system. Comprehensive and practical, ATM Kinase: Methods and Protocols aims to ignite and attract the interest of colleagues from diverse fields to A-T research in an effort to bring their expertise and fresh ideas to resolve many A-T puzzles still waiting to be pieced together and to alleviate the suffering of A-T children and their families.
Series:
Methods in molecular biology, 1064-3745 ; 1599 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.